Effects of endotoxin on expression of ras, p53 and bcl-2 oncoprotein in hepatocarcinogenesis induced by thioacetamide in rats

Table 1 Oncogene ras p21 expression and DNA content in the various study groups

Group	n	FI, x ± s	% cells labeled, x ± s	Cases with positive ras p21expression	DNA index, x ± s
Sham	5	1	17 ± 4	1	1
TAA control	6	1.29 ± 0.085	18 ± 5	6	1.15 ± 0.21
TAA + LPS	6	1.60 ± 0.071a	24 ± 7a	6	1.24 ± 0.25a
TAA + ST	6	1.52 ± 00105a	15 ± 3b	6	1.44 ± 0015ab
TAA + ST + LPS	6	1.32 ± 0.061bc	49 ± 9abc	6	1.56 ± 0.07abc
CBRH-7919 cells	1	1.54	42	1	1.34

^aP < 0.02 vs TAA control group;

^bP < 0.02 vs TAA + LPS group; FI: fluorescence index;

^cP < 0.02 vs TAA + ST group. CBRH-7919: rat hepatocarcinoma cell line; DNA index: ratio of mean nuclear DNA content of the treated rat to mean DNA content of normal rats.

Table 2 p53 expression and hepatocarcinoma rate in the various study groups

Group	n	FI, x ± s	% cells labeled, x ± s	Cases with positive ras p21 expression	DNA index, x ± s
Sham	5	1	32	1	0
TAA control	6	1.15 ± 0.067	22 ± 12	6	17
TAA + LPS	6	1.32 ± 0.024a	20 ± 8.2	6	33
TAA + ST	6	1.22 ± 0.088a	25 ± 12	6	50
TAA + ST + LPS	6	1.29 ± 0.083a	30 ± 14	6	67
CBRH-7919 cells	1	1.35	9	1

^aP < 0.01 vs TAA control group; ^bP < 0.05 vs TAA + LPS group; ^cP < 0.01 vs TAA + ST group. CBRH-7919: rat hepatocarcinoma cell line.

Table 3 Expression of bcl-2 protein and percentage of cells in S and G₂M phases

Group	N	FI, x ± s	% cells labeled, x ± s	Cases with positive ras p21expression	DNA index, x ± s
Sham	5	1	23	1	25 ± 3.2
TAA control	6	0.96 ± 0.14	23 ± 6	4	33 ± 4.7
TAA + LPS	6	1.06 ± 0.07	37 ± 14	6	35 ± 4.5
TAA + ST	6	1.12 ± 0.11	28 ± 14	6	35 ± 7.7
TAA + ST + LPS	6	1.30 ± 0.09abc	25 ± 6	6	45 ± 3.9abc
CBRH-7919 cells	1	1.56	15	1	30

^aP < 0.05 vs TAA control group;

^bP < 0.05 vs TAA + LPS group;

^cP < 0.05 vs TAA + ST group. CBRH-7919: rat hepatocarcinoma cell line.