Analysis of two constitutive forms of microsomal heme oxygenase in different rat tissues

Table 1 Levels of sIL-2R, ALT, and HBV DNA in the sera of patients with chronic HBV infection (mean ± SD)

Purified fractions	Total protein, mg	Total activity, ×103 U	Specific activity, U/mg	Recovery, %	Purification, -fold
HO-1
Solubilized microsomes	82	68.6	837.5	100	1.0
		(64.0-73.2)	(781.4-893.6)
DEAE-Sephacel	8.7	18.3	2097.3	10.7	2.5
		(16.6-20.0)	(1902.9-2291.7)
Hydroxyapatite	1.2	5.1	4301.1	1.5	5.1
		(5.0-5.2)	(3692.8-4909.3)
HO.2
Solubilized microsomes	82	68.6	837.5	100	1.0
		(64.0-73.2)	(781.4-893.6)
DEAE-Sephacel	4.9	7.7	1574	6.0	1.9
		(7.3-8.0)	(1504.5-1643.6)
Hydroxyapatite	3.1	6.7	2146.3	3.8	2.6
		(5.3-8.0)	(1708.5-2584.0)

Table 2 Purification of HO-2 from treated rat brain microsomal fractions

Purified fractions	Total protein,mg	Total activity,×103 U	Specific activity,U/mg	Recovery,%	Purification,-fold
HO-2
Solubilized microsomes	56.0	24	428.7	100	1.0
		(16.9-31.1)	(302.7-554.7)
DEAE-Sephacel	20.1	12.2	607.7	35.9	1.4
		(10.3-14.1)	(512.0-703.4)
Hydroxyapatite	1.6	10.6	6478.1	2.9	15.1
		(10.0-11.2)	(6102.4-6853.8)

Table 3 Purification of HO-2 from intact rat liver microsomal fractions

Purified fractions	Total protein,mg	Total activity,×103 U	Specific activity,U/mg	Recovery,%	Purification,-fold
HO-2
Solubilized microsomes	662.7	394.7	595.6	100	1
		(351.8-437.6)	(530.9-660.3)
DEAE-Sephacel	10.2	16	1574.4	1.5	2.6
		(13.8-18.2)	(1358.7-1789.5)
Hydroxyapatite	2.4	8.2	3381.6	0.4	5.7
		(7.3-9.0)	(3020.1-3743.2)