Construction of retroviral vectors to induce a strong expression of human class interferon gene in human hepatocellular carcinoma cells in vitro

Table 1 Human interferon gene expression in supernatants of gene modified human tumor cells at 20^th day after infection with recombinant retroviruses (x^-± s)

Cell lines	Origin	AFP1	Genetic alteration	G418 resistant	Interferon bioactivity (unit/106 cells·24 h)
HepG2	Human	2044	Parental2	-	0
(n = 5)	hepatocyte		MNSM	+	0
			MNSIFNB	+	218 ± 102
			MNAIFNB	+	818 ± 35
			MNSIFNA	+	128 ± 36
			MNAIFNA	+	808 ± 180
			AMNSIFNA	+	1200 ± 36
Hep3B	Human	311	Parental	-	0
(n = 4)	hepatocyte		MNSM	+	0
			MNSIFNB	+	156 ± 48
			MNAIFNB	+	462 ± 104
			MNSIFNA	+	136 ± 42
			MNAIFNA	+	548 ± 120
			AMNSIFNA	+	465 ± 204
SMMC7721	Human	ND	Parental	-	0
(n = 5)	hepatocyte		MNSM	+	0
			MNSIFNB	+	280 ± 80
			MNAIFNB	+	580 ± 56
			MNSIFNA	+	242 ± 120
			MNAIFNA	+	468 ± 84
			AMNSIFNA	+	524 ± 132
M21	Human		Parental	-	0
(n = 5)	melanocyte		MNSM	+	0
			MNSIFNB	+	324 ± 66
			MNAIFNB	+	21 ± 10
			MNSIFNA	+	226 ± 128
			MNAIFNA	+	42 ± 36
			AMNSIFNA	+	0 ± 2.4
RZ94602	Human renal		Parental	-	0
(n = 4)	cell		MNSM	+	0
			MNSIFNB	+	280 ± 64
			MNAIFNB	+	18 ± 8.4
			MNSIFNA	+	320 ± 120
			MNAIFNA	+	24 ± 48
			AMNSIFNA	+	0 ± 2.4

¹Expressed as ng secreted per mg of cell protein per 4 d, based on historical data[8];

²Unmodified parental tumor cell lines; ND: Undetermined