Poly(A)-specific ribonuclease protein promotes the proliferation, invasion and migration of esophageal cancer cells

Table 1 Poly(A)-specific ribonuclease shRNA target sequence

Target number	Target sequence
Human-PARN-1	TATGACACAGCCTCTGAACA
Human-PARN-2	TGGATACTAAATTGATGGCCA
Human-PARN-3	CAACACATCCCTTGCGGAATT

PARN: Poly(A)-specific ribonuclease.

Table 2 Primer sequences

Gene		Sequence (5’-3’)	Tm (°C)
GAPDH	Forward primer	GAAAGCCTGCCGGTGACTAA	60.32
	Reverse primer	GCCCAATACGACCAAATCAGAG	59.39
PARN	Forward primer	GCCGCGGAATTCGATTTTAAG	58.63
	Reverse primer	ATCGATGGCGAAGAAGTCGG	60.25

PARN: Poly(A)-specific ribonuclease.

Table 3 Relationship between poly(A)-specific ribonuclease expression and tumor characteristics in patients with esophageal cancer

Features	No. of patients	PARN expression		P value
		Low	High
All patients	91	48	43
Age (yr)				0.758
< 65	45	23	22
≥ 65	46	25	21
Gender				0.433
Male	73	40	33
Female	18	8	10
Tumor size				0.110
≤ 5 cm	46	28	18
> 5 cm	35	15	20
T Infiltrate				0.680
T0	1	1	0
T1	3	1	2
T2	15	8	7
T3	39	22	17
T4	10	4	6
Lymphatic metastasis (n)				0.028a
N0	31	20	11
N1	18	10	8
N2	11	4	7
N3	8	2	6
Stage				0.336
I	3	2	1
II	30	18	12
III	33	14	19
IV	2	2	0
Lymphoid positive number				0.164
< 1	43	26	17
≥ 1	46	21	25
Grade				0.516
I	7	4	3
II	49	25	24
III	26	16	10

^aP < 0.05.

The high expression group: Poly(A)-specific ribonuclease immunohistochemistry scores > 6; the low expression group: Poly(A)-specific ribonuclease immunohistochemistry scores ≤ 6. PARN: Poly(A)-specific ribonuclease.

Table 4 Relationship between poly(A)-specific ribonuclease expression and tumor characteristics (lymphatic metastasis) in patients with esophageal cancer (Spearman’s correlation coefficient for ranked data)

		PARN
Lymphatic metastasis (N)	Spearman correlation analysis	0.269
	Significance (two-tailed)	0.027a
	N	68

^aP < 0.05.

PARN: Poly(A)-specific ribonuclease.

Table 5 Immunohistochemistry scoring criteria

Score type	Score point	Score
Positive cell score	No positive signal	0 (negative)
	0% < the proportion of positive cells < 25%	1
	25% ≤ the proportion of positive cells < 50%	2
	50% ≤ the proportion of positive cells < 75%	3
	75% ≤ the proportion of positive cells	4
Staining intensity score (the staining intensity of cytoplasm, membrane or nucleus)	No signal color	0 (negative)
	Pale yellow	1
	Brown yellow	2
	Dark brown	3

The positive cell score × staining color intensity score was used to judge the immunohistochemistry results, and the higher the score, the higher the antibody expression (score 0: Negative, score 1-4: Positive, score 5-8: Positive ++, score 9-12: Positive +++).