Expression of the methylcytosine dioxygenase ten-eleven translocation-2 and connexin 43 in inflammatory bowel disease and colorectal cancer

Table 1 Quantitative polymerase chain reaction human and mouse primer sequences

Gene	Primer sequences, 5’ to 3’	Annealing temperature, °C
hCx43	F: CTTCACTACTTTTAAGCAAAAGAG	52
	R: TCCCTCCAGCAGTTGAG
hTET-1	F: TTCGTCACTGCCAACCTTAG	60
	R: ATGCCTCTTTCACTGGGTG
hTET-2	F: CACTGCATGTTTGGACTTCTG	60
	R: TGCTCATCCTCAGGTTTTCC
hTET-3	F: GCCCACAAGGACCAGCATAA	60
	R: CGCAGCGATTGTCTTCCTTG
hGAPDH	F: TGGTGCTCAGTGTAGCCCAG	52-62
	R: GGACCTGACCTGCCGTCTAG
mCx43	F: ACAGCGGTTGAGTCAGCTTG	60
	R: GAGAGATGGGGAAGGACTTGT
mTET-2	F: CAAGGTGTGCTTGGCAATTTT	60
	R: CATCACTGTGCGTCAATCAAGA
mIl-1β	F: CTCCACCTCAATGGACAGAA	57
	R: GCCGTCTTTCATTACACAGG
mTNF-α	F: AATGGCCTCCCTCTCATCAGT	57
	R: CCACTTGGTGGTTTGCTACGA
mGAPDH	F: CATGGCCTTCCGTGTTCCTA	58
	R: CCTGCTTCACCACCTTCTTGAT

Cx43: Connexin 43; F: Forward; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; h: Human; Il-1β: Interleukin-1β; m: Mouse; R: Reverse; TET: Ten-eleven translocation; TNF-α: Tumor necrosis factor-α.