P2X7 receptor blockade decreases inflammation, apoptosis, and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice

doi:10.3748/wjg.v28.i30.4075

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Aug 14, 2022 (publication date) through May 5, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Aug 14, 2022; 28(30): 4075-4088
Published online Aug 14, 2022. doi: 10.3748/wjg.v28.i30.4075

Table 1 Primary and secondary antibodies used

Antigen	Host	Dilution	Manufacturer
nNOS	Sheep	1:2000	Millipore (AB1529/Lot 2488802)
ChAT	Goat	1:50	Millipore (AB144P/Lot 1978747)
P2X7 receptor	Rabbit	1:100	Millipore (AB5246/Lot 2361386)
Calretinin	Goat	1:100	Molecular
Donkey anti-rabbit IgG Alexa 594	Donkey	1:200	Probes (A21206/Lot 1182675)
Donkey anti-sheep IgG Alexa 488	Donkey	1:400	Molecular
Donkey anti-sheep IgG Alexa 488	Donkey	1:400	Probes (A11016/Lot93D1-1)

nNOS: Neuronal nitric oxide synthase; ChAT: Choline acetyltransferase; IgG: Immunoglobulin G.

Citation: Santos AAQA, Costa DVS, Foschetti DA, Duarte ASG, Martins CS, Soares PMG, Castelucci P, Brito GAC. P2X7 receptor blockade decreases inflammation, apoptosis, and enteric neuron loss during Clostridioides difficile toxin A-induced ileitis in mice. World J Gastroenterol 2022; 28(30): 4075-4088
URL: https://www.wjgnet.com/1007-9327/full/v28/i30/4075.htm
DOI: https://dx.doi.org/10.3748/wjg.v28.i30.4075