Hepatitis B core antigen modulates exosomal miR-135a to target vesicle-associated membrane protein 2 promoting chemoresistance in hepatocellular carcinoma

Table 1 Clinical sample information

Characteristics	Total	miR-135a-5p		P value
		Negative	Positive
Age (yr)
< 55	12	3	9	0.82
≥ 55	6	2	4
Gender
Male	8	1	7	0.291
Female	10	3	7
Hepatitis B s antigen
Negative	-
Positive	18	5	13	-

Table 2 Sequences and primers for vector construction

Category	Sequence (5’-3’)
miR-135a-5p mimic	F-UAUGGCUUUUUAUUCCUAUGUGA
	R-UCACAUAGGAAUAAAAAGCCAUA
miR-135a-5p inhibitor	UCACAUAGGAAUAAAAAGCCAUA
VAMP2 (NM_001330125.1)	F-CTAGCTAGCATGGACAGGTCTGCTAC
	R-CGCGGATCCTTAAGTGCTGAAGT
VAMP2 3’-UTR-WT	F-CTAGCTAGCATCCCCGAGGAGTCT
	R-ACGCGTCGACAGAGAGGGGTGAAG
VAMP2 3’-UTR-MUT	F-GTTCCTCCACCTCTCACGTGCATCTTTCAGCC CC
	R-GGGGCTGAAAGATGCACGTGAGAGGTGGAGGAAC
Hepatitis B virus-1903/2454	F-CTAGCTAGCGCCACCATGGACATCGACCCTT
	R-CCGCTCGAGCTAACATTGAGATTCCCGAGAT

VAMP2: Vesicle-associated membrane protein 2; WT: Wild-type; MUT: Mutant.

Table 3 Primers for quantitative polymerase chain reaction

Primer	Product size	Sequence (5’-3’)
VAMP2 (NM_001330125.1)	182 bp	F-GGTCTCTCCTGCGTTCCC
		R-TCGACCCGAAAAGACAGGC
GAPDH (NM_002046.7)	197 bp	F-GGAGCGAGATCCCTCCAAAAT
		R-GGCTGTTGTCATACTTCTCATGG
U6 (NR_004394.1)	94 bp	F-CTCGCTTCGGCAGCACA
		R-AACGCTTCACGAATTTGCGT
miR135a (NR_029677.1)	68 bp	F-ACACTCCAGCTGGGTATGGCTTTTTATTCCT
		R-TGGTGTCGTGGAGTCG

VAMP2: Vesicle-associated membrane protein 2; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.