Leucine-rich repeat-containing G protein-coupled receptor 5 marks different cancer stem cell compartments in human Caco-2 and LoVo colon cancer lines

doi:10.3748/wjg.v27.i15.1578

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Apr 21, 2021 (publication date) through Nov 19, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Apr 21, 2021; 27(15): 1578-1594
Published online Apr 21, 2021. doi: 10.3748/wjg.v27.i15.1578

Table 1 Primer sequences

Gene	Forward primer 5’-3’	Reverse primer 5’-3’
GAPDH	TGAAGCTGGAGAAGGAGAAG	ATCGGCCTGTGTATATCCC
LGR5	CCTCTGCTGGCTTTTAGGTG	AGCAGTTTTCAGGCCTTTCA
OLFM4	ACTGTCCGAATTGACATCATGG	TTCTGAGCTTCCACCAAAACTC
ASCL2	ACCTGCGTACCTTGCTTTGG	GAAATCTGCGAGTTCCCGGT
Amplification/cloning of the LGR5 promoter element	CATATGCTAGCTCGAGCTCACTTCGACTTCCTCACCCCGC	AAGCTTGGTGCCCGAAGTAGGGGGCCA

Citation: Alharbi SA, Ovchinnikov DA, Wolvetang E. Leucine-rich repeat-containing G protein-coupled receptor 5 marks different cancer stem cell compartments in human Caco-2 and LoVo colon cancer lines. World J Gastroenterol 2021; 27(15): 1578-1594
URL: https://www.wjgnet.com/1007-9327/full/v27/i15/1578.htm
DOI: https://dx.doi.org/10.3748/wjg.v27.i15.1578