Pediatric non-alcoholic fatty liver disease and kidney function: Effect of HSD17B13 variant

Table 1 Main features in patients with non-alcoholic fatty liver disease according to HSD17B13 genotypes

	HSD17B13 TT (n = 218)	HSD17B13 TA/AA (n = 100)	P value
Age, yr	10.72 ± 2.88	10.60 ± 2.80	0.67
BMI-SDS	2.98 ± 0.73	3.00 ± 0.75	0.85
Duration of obesity, yr	4.65 ± 1.93	4.64 ± 2.17	0.60
Sex (male), %	53.3	51.2	0.82
SBP-SDS	1.00 ± 1.15	1.12 ± 1.14	0.33
DBP-SDS	0.25 ± 0.89	0.30 ± 0.91	0.54
W/Hr	0.62 ± 0.05	0.63 ± 0.06	0.04
ALT, U/L	35.45 ± 21.69	30.60 ± 21.52	0.02
AST, U/L	26.86 ± 10.18	24.49 ± 8.86	0.01
GGT, U/L	20.42 ± 9.73	19.70 ± 9.49	0.45
Total-Cholesterol, mg/dL	160.13 ± 33.85	158.19 ± 30.80	0.55
LDL, mg/dL	95.84 ± 29.31	93.38 ± 23.93	0.40
HDL, mg/dL	44.07 ± 10.68	45.30 ± 11.24	0.67
Triglycerides, mg/dL	101.21 ± 51.36	99.92 ± 47.62	0.79
Glycaemia, mg/dL	83.05 ± 9.10	81.80 ± 8.44	0.14
HOMA-IR	6.19 ± 5.35	5.94 ± 3.86	0.62
eGFR, mL/min/1.73m2	186.71 ± 36.55	197.45 ± 34.92	0.01

ALT: Alanine transaminase; AST: Aspartate transaminase; BMI: Body mass index; DBP: Diastolic blood pressure; eGFR: Estimated glomerular filtration rate; GLM: General linear model; HDL-C: High-density lipoprotein cholesterol; HOMA: Homeostasis model assessment; LDL-C: Low density lipoprotein cholesterol; SBP: Systolic blood pressure; SDS: Standard deviation score; W/Hr: Waist to height ratio.

Table 2 Main features in patients without non-alcoholic fatty liver disease according to HSD17B13 genotypes

	HSD17B13 TT (n = 199)	HSD17B13 TA/AA (n = 167)	P value
Age, yr	10.36 ± 2.98	10.36 ± 3.20	0.89
Duration of obesity	2.96 ± 0.79	2.95 ± 0.96	0.94
BMI -SDS	4.41 ± 1.84	4.88 ± 2.51	0.24
Sex (male), %	50.5	55.5	0.68
SBP-SDS	1.08 ± 1.24	0.79 ± 1.20	0.09
DBP-SDS	0.18 ± 0.75	1.03 ± 1.15	0.64
W/Hr	0.61 ± 0.05	0.60 ± 0.05	0.21
ALT, U/L	22.95 ± 6.90	20.30 ± 6.56	0.003
AST, U/L	23.03 ± 4.92	21.35 ± 6.07	0.004
GGT, U/L	16.70 ± 5.80	17.38 ± 4.34	0.33
Total-Cholesterol, mg/dL	160.66 ± 27.11	161.84 ± 33.11	0.76
LDL-C, mg/dL	93.74 ± 24.59	98.10 ± 28.27	0.36
HDL-C, mg/dL	48.18 ± 15.28	45.96 ± 8.97	0.50
Triglycerides, mg/dL	96.90 ± 59.74	95.77 ± 38.79	0.87
Glycaemia, mg/dL	79.95 ± 7.34	80.52 ± 7.66	0.08
HOMA-IR	4.63 ± 3.16	5.16 ± 3.75	0.27
eGFR, mL/min/1.73 m2	187.34 ± 37.76	197.82 ± 39.78	0.007

ALT: Alanine transaminase; AST: Aspartate transaminase; BMI: Body mass index; DBP: Diastolic blood pressure; eGFR: Estimated glomerular filtration rate; HDL-C: High-density lipoprotein cholesterol; HOMA: Homeostasis model assessment; LDL-C: Low density lipoprotein cholesterol; SBP: Systolic blood pressure; SDS: Standard deviation score; W/Hr: Waist to height ratio.

Table 3 General linear model for analysis of variance of estimated glomerular filtration rate both among patients with and without non-alcoholic fatty liver disease

Source	Patients with NAFLD			Patients without NAFLD
	Coefficient	F-ratio	P value	Coefficient	F-ratio	P value
Model		6.24	< 0.0001		3.30	0.001
Gender	-0.78	0.62	0.43	0.31	0.09	0.75
Duration of obesity	-2.48	6.17	0.01	-0.47	0.22	0.63
HSD17B13 genotype	1.89	42.58	< 0.0001	4.63	21.49	< 0.0001
PNPLA3 genotype	-3.80	11.19	0.001	-0.90	0.81	0.36
TM6SF2 genotype	1.13	11.82	0.001	1.40	1.97	0.16
BMI-SDS	0.62	0.38	0.53	0.51	0.26	0.61
HOMA-IR	-1.20	1.44	0.23	0.63	0.40	0.52
Triglycerides	-0.94	0.89	0.34	0.95	0.90	0.34
LDL-Cholesterol	0.69	0.47	0.49	0.23	0.05	0.81

NAFLD: Model r2 0.39, adjusted r2 0.33; Non NAFLD: Model r2 0.16, adjusted r2 0.11. ALT: Alanine transaminase; BMI: Body mass index; eGFR: Estimated glomerular filtration rate; GLM: General linear model; HOMA: Homeostasis model assessment; LDL: Low density lipoprotein; SDS: Standard deviation score.