Liver structural transformation after partial hepatectomy and repeated partial hepatectomy in rats: A renewed view on liver regeneration

Table 1 Distribution of the experimental animals by experimental models and research methods

Methods Groups	Histology					SEM of corrosion cast	Ploidy of hepatocytes	Number of animals
	H&E	Histochemistry(Masson’s Trichrome)	IHC (CK8)	Morphometry(perimeters and areas of the hepatocytes and their nuclei)	Transparent slides after Indian Ink injection
Control	6*	6*	6*	6*	4	6	3*	16
Study group 1 (9 mo after PH)	6**	6**	6**	6**	4	4	3**	14
Study group 2 (6 mo after repeated PH conducted after 9 mo from primary intervention)	4***	4***	4***	4***	2	4	3***	10
Total 40

Note: A similar number of (*) indicates that the same animals were used for different research methods. PH: Partial hepatectomy; SEM: Scanning electron microscopy.

Table 2 Data of the morphometric study of hepatocyte areas and perimeters of the 1^st and 3^rd zones of liver acini of control group, study group 1 and study group 2

Study groups	III zone (mean ± SD)		I zone (mean ± SD)
	Area	Perimeter	Area	Perimeter
Control group (CG)	283 ± 88	64 ± 10	255 ± 66	62 ± 8
Study group 1 (SG1)	331 ± 95	71 ± 11	348 ± 90	71 ± 10
Study group 2 (SG2)	390 ± 128	75 ± 11	372 ± 107	73 ± 11

All measurements are carried out and presented in micrometers. Area, I zone of control group (CG) vs III zone of CG, P < 0.05; Perimeter, I zone CG vs III zone CG, P > 0.05. Area, III zone CG vs study group 1 (SG₁), P < 0.05; Perimeter, III zone CG vs SG₁, P value < 0.05. Perimeter, I zone CG vs SG₁, P < 0.05. Area, I zone SG₁vs III zone SG₁, P > 0.05; Perimeter, I zone SG₁vs III zone SG₁, P > 0.05. Area, III zone CG vs study group 2 (SG₂), P < 0.05; Perimeter, III zone CG vs SG₂, P < 0.05. Area, III zone SG₁vs SG₂, P < 0.05; Perimeter, III zone SG₁vs SG₂, P > 0.05. Area, I zone SG₁vs SG₂, P > 0.05; Perimeter, I zone SG₁vs SG₂; P value > 0.05. Area, I zone SG₂vs III zone SG₂, P > 0.05; Perimeter, I zone SG₂vs III zone SG₂, P > 0.05.