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©The Author(s) 2020.
World J Gastroenterol. Mar 14, 2020; 26(10): 1067-1079
Published online Mar 14, 2020. doi: 10.3748/wjg.v26.i10.1067
Published online Mar 14, 2020. doi: 10.3748/wjg.v26.i10.1067
Table 1 Characteristics of 432 hepatitis B virus-infected patients in different fibrosis stages diagnosed by liver biopsy (mean ± SD)
Variables | Fibrosis stage | P value | |||
F0-F1 | F2 | F3 | F4 | ||
Cases, n | 95 | 126 | 103 | 108 | / |
Male, n (%) | 62 (65.26) | 88 (69.84) | 82 (79.61) | 89 (82.41) | / |
Age (yr) | 37.91 ± 10.71 | 36.52 ± 11.11 | 41.71 ± 10.01 | 46.63 ± 9.95 | < 0.001c |
HBV DNA (IU/mL) | 1.26 × 108 ± 3.64 ×108 | 1.33 × 108 ± 4.01 × 108 | 6.39 × 107 ± 1.62 × 108 | 5.63 × 107 ± 1.56 × 108 | 0.229 |
AFP (ng/mL) | 3.20 ± 3.01 | 19.37 ± 100.27 | 12.56 ± 23.25 | 40.98 ± 160.29 | 0.118 |
ALT (IU/L) | 42.58 ± 61.49 | 125.21 ± 211.29 | 263.82 ± 421.57 | 115.84 ± 165.90 | < 0.001c |
AST (IU/L) | 30.45 ± 39.36 | 85.65 ± 171.17 | 185.61 ± 292.67 | 92.18 ± 127.81 | < 0.001c |
GGT (IU/L) | 31.59 ± 26.74 | 42.10 ± 50.37 | 78.13 ± 107.06 | 64.47 ± 68.93 | < 0.001c |
ALP (IU/L) | 75.63 ± 21.27 | 81.56 ± 31.47 | 109.30 ± 120.10 | 103.82 ± 41.55 | 0.002b |
PLT (109/L) | 206.91 ± 51.14 | 198.36 ± 55.72 | 185.36 ± 58.29 | 134.95 ± 46.41 | < 0.001c |
Total bilirubin (μmol/L) | 13.09 ± 5.30 | 18.34 ± 16.99 | 29.93 ± 31.44 | 22.67 ± 12.60 | < 0.001c |
Indirect bilirubin (μmol /L) | 7.78 ± 4.31 | 8.40 ± 9.40 | 13.46 ± 17.05 | 10.01 ± 7.94 | 0.003b |
Total protein (g/L) | 73.46 ± 4.78 | 73.61 ± 10.53 | 72.48 ± 11.14 | 74.44 ± 7.66 | 0.635 |
Albumin (g/L) | 45.80 ± 3.30 | 45.44 ± 4.40 | 45.33 ± 6.51 | 44.39 ± 5.39 | 0.320 |
Table 2 Inflammation grade and fibrosis stage of 432 hepatitis B virus-infected patients
Fibrosis stage | Inflammation grade | |||||
G0 | G1 | G2 | G3 | G4 | Total | |
F0-F1 | 8 | 71 | 16 | 0 | 0 | 95 |
F2 | 0 | 31 | 83 | 12 | 0 | 126 |
F3 | 0 | 12 | 40 | 47 | 4 | 103 |
F4 | 0 | 11 | 38 | 55 | 4 | 108 |
Total | 8 | 125 | 177 | 114 | 8 | 432 |
Table 3 N-glycome profiling results in hepatitis B virus-infected patients with liver fibrosis F0-F4 and differences between different fibrosis stages in the modeling group (mean ± SD)
Peak | F0-F1 | F2 | F3 | F4 | F0-F1 vs F2-F4 | F0-F2 vs F3-F4 | F0-F3 vs F4 |
1 | 5.92 ± 1.46 | 7.62 ± 2.57 | 7.51 ± 2.09 | 8.68 ± 3.60 | < 0.001c | < 0.001c | < 0.001c |
2 | 1.95 ± 0.67 | 1.78 ± 0.76 | 1.60 ± 0.57 | 1.85 ± 0.76 | 0.161 | 0.235 | 0.312 |
3 | 4.57 ± 1.16 | 5.96 ± 1.43 | 6.04 ± 1.17 | 6.21 ± 1.66 | < 0.001c | < 0.001c | 0.003b |
4 | 7.34 ± 1.37 | 5.96 ± 1.15 | 5.54 ± 0.81 | 5.43 ± 0.81 | < 0.001c | < 0.001c | < 0.001c |
5 | 47.90 ± 3.77 | 46.93 ± 4.64 | 46.75 ± 4.59 | 46.27 ± 6.23 | 0.097 | 0.163 | 0.197 |
6 | 16.89 ± 2.92 | 17.66 ± 3.01 | 18.13 ± 2.89 | 17.30 ± 2.58 | 0.086 | 0.323 | 0.413 |
7 | 4.48 ± 1.08 | 4.53 ± 0.95 | 4.86 ± 1.22 | 4.90 ± 1.16 | 0.089 | 0.004b | 0.058 |
8 | 8.07 ± 2.07 | 6.91 ± 1.90 | 6.74 ± 1.70 | 6.22 ± 2.16 | < 0.001c | < 0.001c | 0.001b |
9 | 2.87 ± 1.27 | 2.65 ± 1.01 | 2.82 ± 1.12 | 3.12 ± 1.33 | 0.684 | 0.136 | 0.035a |
Table 4 Predictive value of fibrosis diagnostic models in the modeling group
Fibrosis stage | Model | Cutoff value | Sen | Spec | PPV | NPV | AUROC |
F0-F1 vs F2-F4 | Model A | 0.8032 | 0.871 | 0.839 | 0.951 | 0.642 | 0.890 |
GlycoFibroTest | -0.4404 | 0.280 | 0.903 | 0.913 | 0.257 | 0.538 | |
GlycoCirrhoTest | -0.2946 | 0.778 | 0.452 | 0.837 | 0.359 | 0.644 | |
APRI | 0.4871 | 0.626 | 0.839 | 0.933 | 0.385 | 0.790 | |
FIB-4 | 0.4720 | 0.401 | 0.855 | 0.908 | 0.285 | 0.599 | |
S index | 0.0543 | 0.742 | 0.596 | 0.871 | 0.386 | 0.701 | |
Model A + ALT + PLT | 0.7779 | 0.923 | 0.806 | 0.945 | 0.746 | 0.912 | |
F0-F2 vs F3-F4 | Model B | 0.3418 | 0.986 | 0.514 | 0.662 | 0.974 | 0.752 |
GlycoFibroTest | -0.2295 | 0.128 | 1.000 | 1.000 | 0.543 | 0.524 | |
GlycoCirrhoTest | 0.0033 | 0.270 | 0.911 | 0.745 | 0.564 | 0.618 | |
APRI | 0.3964 | 0.819 | 0.562 | 0.638 | 0.766 | 0.740 | |
FIB-4 | 0.3930 | 0.609 | 0.726 | 0.677 | 0.663 | 0.699 | |
S index | 0.0890 | 0.664 | 0.711 | 0.690 | 0.686 | 0.738 | |
Model B + ALT + PLT | 0.4404 | 0.855 | 0.685 | 0.720 | 0.833 | 0.829 | |
F0-F3 vs F4 | Model C | 0.2258 | 0.808 | 0.598 | 0.407 | 0.901 | 0.747 |
GlycoFibroTest | -0.3200 | 0.274 | 0.939 | 0.606 | 0.791 | 0.571 | |
GlycoCirrhoTest | -0.0287 | 0.356 | 0.827 | 0.413 | 0.790 | 0.607 | |
APRI | 0.4206 | 0.859 | 0.488 | 0.359 | 0.912 | 0.666 | |
FIB-4 | 0.3762 | 0.831 | 0.657 | 0.447 | 0.921 | 0.795 | |
S index | 0.0817 | 0.765 | 0.571 | 0.380 | 0.876 | 0.717 | |
Model C + ALT + PLT | 0.3010 | 0.789 | 0.845 | 0.629 | 0.923 | 0.885 |
- Citation: Cao X, Shang QH, Chi XL, Zhang W, Xiao HM, Sun MM, Chen G, An Y, Lv CL, Wang L, Nan YM, Chen CY, Tan ZN, Liu XE, Zhuang H. Serum N-glycan markers for diagnosing liver fibrosis induced by hepatitis B virus. World J Gastroenterol 2020; 26(10): 1067-1079
- URL: https://www.wjgnet.com/1007-9327/full/v26/i10/1067.htm
- DOI: https://dx.doi.org/10.3748/wjg.v26.i10.1067