Basic Study
Copyright ©The Author(s) 2020.
World J Gastroenterol. Mar 14, 2020; 26(10): 1029-1041
Published online Mar 14, 2020. doi: 10.3748/wjg.v26.i10.1029
Table 1 Primers used for quantitative real-time PCR experiments
GeneSpeciesPrimers
MANFHomo sapiensF: 5’-CGGTTGTGCTACTATATCGGGG-3’
R: 5’-GGCCAGAGGCTTTGATACCT-3’
ACCαHomo sapiensF: 5’-ATGTCTGGCTTGCACCTAGTA-3’
R: 5’-CCCCAAAGCGAGTAACAAATTCT-3’
FASHomo sapiensF: 5’-AGATTGTGTGATGAAGGACATGG-3’
R: 5’-TGTTGCTGGTGAGTGTGCATT-3’
SREBP-1CHomo sapiensF: 5’-CGGAACCATCTTGGCAACAGT-3’
R: 5’-CGCTTCTCAATGGCGTTGT-3’
HMGCRHomo sapiensF: 5’-TGATTGACCTTTCCAGAGCAAG-3’
R: 5’-CTAAAATTGCCATTCCACGAGC-3’
CD36Homo sapiensF: 5’-GGCTGTGACCGGAACTGTG-3’
R: 5’-AGGTCTCCAACTGGCATTAGAA-3’
FABP-1Homo sapiensF: 5’-GTGTCGGAAATCGTGCAGAAT-3’
R: 5’-GACTTTCTCCCCTGTCATTGTC-3’
β-actinHomo sapiensF: 5’-GCCGACAGGATGCAGAAGG-3’
R: 5’-TGGAAGGTGGACAGCGAGG-3’
MANFMus musculusF: 5’-TCTGGGACGATTTTACCAGGA-3’
R: 5’-TCTTGCTTCACGGCAAAACTTTA-3’
β-actinMus musculusF: 5’-TCACTGTCCACCTTCCAGCAGATG-3’
R: 5’-CTCAGTAACAGTCCGCCTAGAAGC-3’