Effect of Sheng-jiang powder on multiple-organ inflammatory injury in acute pancreatitis in rats fed a high-fat diet

Table 1 Feeding schedules used in the present study

Group	Rats (n)	Food	Treatment (oral administration for 10 wk)
CG	8	Control diet	Equal volumes of normal saline.
OG	8	High-fat diet	Equal volumes of normal saline.
SG	8	High-fat diet	Sheng-jiang powder (5 g/kg of body weight)

CG: Control group; OG: Obese group; SG: Sheng-jiang powder treatment group.

Table 2 Compositions of the experimental diet

Ingredient	Control diet (LAD3001G)		High-fat diet (TP23300)
	g	kcal	g	kcal
Casein	200	753.20	267	1335
Corn starch	397	1495.10	0	0
Maltodextrin	132	497.11	157	785
Sucrose	100	376.60	89	445
Soybean oil	70	263.62	33	165
Lard	0	0.00	301	1505
Cellulose	50	188.30	67	335
Mineral mixture	35	131.81	66	330
Vitamin mixture	10	37.66	13	65
L-cystine	3	11.30	4	20
Choline bitartrate	0	0.00	3	15
Choline chloride	3	11.30	0	0
Total	1000	3766	1000	5000

Table 3 Body weight, Lee's index, and serum biochemistry parameter levels of rats

Parameter	CG	OG	SG
Body weight (g)	480.10 ± 43.62	608.10 ± 36.96b	536.60 ± 46.77d
Lee’s index	301.40 ± 23.36	332.90 ± 19.52a	307.30 ± 19.40b
TG (mmol/L)	1.35 ± 0.51	2.69 ± 0.86b	1.60 ± 0.60d
TC (mmol/L)	1.46 ± 0.32	1.97 ± 0.21b	1.68 ± 0.19b
HDL-c (mmol/L)	0.68 ± 0.11	0.46 ± 0.07b	0.59 ± 0.12b
LDL-c (mmol/L)	0.54 ± 0.13	0.49 ± 0.11	0.48 ± 0.11

The results are presented as the mean ± SD.

^aP < 0.05.

^bP < 0.01 vs the control group.

^cP < 0.05.

^dP < 0.01 vs the obese group.

CG: Control group; OG: Obese group; SG: Sheng-jiang powder treatment group; TG: Triglyceride; TC: Total cholesterol; HDL-c: High-density lipoprotein cholesterol; LDL-c: Low-density lipoprotein cholesterol.

Table 4 Oxidative stress conditions of multiple organs

Organ	CG	OG	SG
Pancreas
MDA (pmol/mL)	1048 ± 116	1584 ± 278b	1066 ± 156d
SOD (U/mL)	338 ± 31	244±21b	285 ± 18d
Heart
MDA (pmol/mL)	1358 ± 199	1685 ± 87b	1297 ± 83d
SOD (U/mL)	313 ± 24	321 ± 42	351 ± 14
Spleen
MDA (pmol/mL)	1247 ± 205	1748 ± 96b	1569 ± 185b
SOD (U/mL)	288 ± 17	214 ± 15b	322 ± 44d
Lung
MDA (pmol/mL)	1359 ± 283	1745 ± 86a	1282 ± 140d
SOD (U/mL)	153 ± 31	268 ± 21b	241 ± 23
MPO (ng/mL)	77 ± 5	79 ± 12	56 ± 6b
Intestine
MDA (pmol/mL)	1266 ± 141	1412 ± 325	1414 ± 235
SOD (U/mL)	347 ± 12	240 ± 19b	341 ± 29d
MPO (ng/mL)	46 ± 3	75 ± 9b	64 ± 3
Liver
MDA (pmol/mL)	1285 ± 146	1549 ± 92b	1235 ± 160d
SOD (U/mL)	308 ± 47	229 ± 11b	231 ± 12
ROS (IU/mL)	718 ± 146	819 ± 96	730 ± 85
GSH-Px (mIU/mL)	86 ± 11	71 ± 20	80 ± 6
Kidney
MDA (pmol/mL)	1077 ± 236	1519 ± 217b	1513 ± 223
SOD (U/mL)	240 ± 22	350 ± 22b	316 ± 55
ROS (IU/mL)	598 ± 85	677 ± 142	666 ± 49
GSH-Px (mIU/mL)	53 ± 7	66 ± 17	82 ± 13b

The results are presented as the mean ± SD.

^aP < 0.05.

^bP < 0.01 vs the control group; ^cP < 0.05.

^dP < 0.01 vs the obese group.

CG: Control group; OG: Obese group; SG: Sheng-jiang powder treatment group; MDA: Malondialdehyde; SOD: Superoxide dismutase; MPO: Myeloperoxidase; ROS: Reactive oxygen species; GSH-Px: Glutathione peroxidase.