Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice

Table 1 PCR primers for chimeric mouse genotyping and corresponding band results

Sequence (5’-3’)	Results
Cldn7 loxP-tF primer : GTACGAGTTTGGACCT GCCA	Fln/Fln = 493 bp; Fln/wt = 493/375 bp; wt/wt = 375 bp
Cldn7-LoxP-tR primer : TGTGCAA GGATCTGGGTCTG
Cldn7-FRT-tF primer : CTGATCTGGGTGTCCCACGT	Fln/Fln = 507 bp; Fln/wt = 507/0 bp; wt/wt = 0bp
ZMK-2F4 primer: GCATCGCATTGTCTGAG TAGGTG
Neo-5R primer : GGCTGG ACGTAAACTCCTC	Fln/Fln = 259 bp; Fln/wt = 259/0bp; wt/wt = 0 bp
Cldn7-FRT-tR primer: CGATGAAACC GTTCCAGGTA

Table 2 PCR primers for gene knockout mouse genotyping and corresponding explanations

Primer sequence (5’-3’)	Results	Description
Cldn7-FRTtF2: CCTGGGATCTGATCTGGGTG	wt = 600 bp, fln = none; fl = 756 bp, Null = none	Check if flper is reorganized
Cldn7-FRTtR2: GGCAGGTAGCCTTAGGATGG
ZMK2F4: GCATCGCATTGTCTGAGTAGGTG	wt = none, fln = 515 bp; fl = none, Null = none	Check if Neo is deleted completely
Cldn7-FRTtF2: CCTGGGATCTGATCTGGGTG
Cldn7-loxPtF2: CTTGGGAGACATCAGGTCCG	wt = 512 bp, fln = 630 bp; fl = 630 bp, Null = none	Homozygous identification
Cldn7-loxPtR2: GAGGCAATAGGCCCAAGGAG
Cldn7-FRTtF2: CCTGGGATCTGATCTGGGTG	wt = none, fln = none; fl = none, Null = 640 bp	Check if Cre mediated recombination occurs
Cldn7-loxPtR2: GAGGCAATAGGCCCAAGGAG
Cre-up: GCCTGCATTACCGGTCGATGC	T: 481 bp; W: none	Detect Cre
Cre-low: CAGGGTGTTATAAGCAATCCC