Copyright
©The Author(s) 2017.
World J Gastroenterol. Aug 21, 2017; 23(31): 5692-5699
Published online Aug 21, 2017. doi: 10.3748/wjg.v23.i31.5692
Published online Aug 21, 2017. doi: 10.3748/wjg.v23.i31.5692
Table 1 Primer sequences used in gene expression studies
| Target gene | Primer sequence (5’-3’) |
| 18s | Forward: 5’-GTAACCCGTTGAACCCCATTC-3’ |
| Reverse: 5’-GCCTCACTAAACCATCCAATC G-3’ | |
| TGFβ | Forward: 5’-TGCCCTCTACAACCAACACA-3’ |
| Reverse: 5’-GTTGGACAACTGCTCCACCT-3 | |
| MMP-2 | Forward: 5’-ACCCAGATGTGGCCAACTAC-3’ |
| Reverse: 5’-TCATTTTAAGGCCCGAGCAA-3’ | |
| TIMP-1 | Forward: 5’-ACGAGACCACCTTATACCAGCCG-3’ |
| Reverse: 5’-GCGGTTCTGGGACTTGTGGGC-3’ | |
| PAR-1 | Forward: 5’-CTCCTCAAGGAGCAGACCCAC-3’ |
| Reverse: 5’-AGACCGTGGAAACGATCAAC-3’ | |
| PAR-2 | Mm00433160_m1, using TaqMan Gene Expression Master Mix (Applied Biosystems) |
| 18S | Hs03003631_g1 using TaqMan Gene Expression Master Mix (Applied Biosystems) |
- Citation: Knight V, Lourensz D, Tchongue J, Correia J, Tipping P, Sievert W. Cytoplasmic domain of tissue factor promotes liver fibrosis in mice. World J Gastroenterol 2017; 23(31): 5692-5699
- URL: https://www.wjgnet.com/1007-9327/full/v23/i31/5692.htm
- DOI: https://dx.doi.org/10.3748/wjg.v23.i31.5692