Solid lipid nanoparticles delivering anti-inflammatory drugs to treat inflammatory bowel disease: Effects in an in vivo model

Table 1 Composition of warm microemulsion for cholesteryl butyrate and dexamethasone cholesteryl butyrate-solid lipid nanoparticles formulations

Molar composition of warm microemulsion	Cb, mmol/L	DxCb-SLN, mmol/L
Dexamethasone 21-acetate	-	8.1
Cholesteryl butyrate	273.7	273.7
Epikuron™ 200 (purified phosphatidylcholine 92%)	335.5	335.5
Sodium glycocholate	194.8	194.8

Cb: Cholesteryl butyrate; DxCb-SLN: Dexamethasone cholesteryl butyrate-solid lipid nanoparticles.

Table 2 Concentration of main components of water dispersion of cholesteryl butyrate and dexamethasone cholesteryl butyrate-solid lipid nanoparticles formulations after 4 washing steps (HPLC method determination)

Molar composition of final dispersion after washing	Cb, mmol/L	DxCb-SLN, mmol/L
Dexamethasone 21-acetate	-	1.1
Cholesteryl butyrate	36.0	38.5
Phosphatidylcholine	48.4	49.2
Sodium glycocholate	12.5	11.2

Cb: Cholesteryl butyrate; DxCb-SLN: Dexamethasone cholesteryl butyrate-solid lipid nanoparticles.

Table 3 Average value of hydrodynamic diameter (Zave) and polidispersity index of cholesteryl butyrate and dexamethasone cholesteryl butyrate-solid lipid nanoparticles formulations

Physical characterization DLS analysis	Cb	DxCb-SLN
Zave in nm	79.6	72.9
Polydispersity index	0.25	0.28

Cb: Cholesteryl butyrate; DxCb-SLN: Dexamethasone cholesteryl butyrate-solid lipid nanoparticles.