Effects of endoplasmic reticulum stress on the expression of inflammatory cytokines in patients with ulcerative colitis

Table 1 Clinical data of patients with ulcerative colitis

No.	Gender	Age (yr)	Enteroscopic diagnosis	Pathological appearance
1	M	25	Severe, in acute period	Acute and chronic inflammation
2	M	52	Severe, in acute period	Acute and chronic inflammation
3	F	48	Moderate-severe, in acute period	Chronic and chronic inflammation
4	M	43	Moderate-severe, in remission period	Chronic inflammation
5	F	57	Moderate, in acute period	Chronic inflammation
6	M	50	Moderate, in acute period	Chronic inflammation
7	M	42	Moderate, in remission period	Chronic inflammation
8	M	23	Moderate, in acute period	Acute and chronic inflammation
9	M	41	Moderate -severe, in acute period	Acute and chronic inflammation
10	M	23	Moderate, in acute period	Acute inflammation
11	M	45	Moderate-severe, in acute period	Acute and chronic inflammation
12	M	35	Severe, in remission period	Acute and chronic inflammation
13	M	47	Severe, in acute period	Acute and chronic inflammation
14	M	33	Moderate -severe, in remission period	Chronic inflammation
15	F	56	Moderate, in remission period	Chronic inflammation
16	M	51	Moderate, in acute period	Chronic inflammation
17	M	40	Moderate, in remission period	Acute inflammation
18	M	22	Moderate, in acute period	Acute and chronic inflammation
19	M	39	Moderate-severe, in remission period	Chronic inflammation
20	M	27	Moderate, in acute period	Acute inflammation
21	M	43	Moderate-severe, in remission period	Acute inflammation

Table 2 Primer sequences of human cytokines

Human cytokines	Primer name	Sequence
IL-2	Forward	5’-AAGTTTTACATGCCCAAGAAGG-3’
	Reverse	5’-AAGTGAAAGTTTTTGCTTTGAGCTA-3’
IFN-γ	Forward	5’-AGGGAAGCGAAAAAGGAGTCA-3’
	Reverse	5’-GGACAACCATTACTGGGATGCT-3’
IL-17α	Forward	5’-GAGCCCCAAAAGCAAGAGGAA-3’
	Reverse	5’-TGCGGGCATACGGTTTCATC-3’
IL-4	Forward	5’-GCCAAGACCCCTTCGAGAAAT-3’
	Reverse	5’-CCGTCCCTGTTATCTGCCTCC-3’
GAPDH	Forward	5’-TGTGGGCATCAATGGATTTGG-3’
	Reverse	5’-ACACCATGTTATTCCGGGTCAAT-3’

IL: Interleukin; IFN: Interferon.

Table 3 Primer sequences of X-box binding protein 1 splicing

XBP1	Primer name	Sequence
Human XBP1	Forward	5’-A AAC AG A GTA GCA GCT CAG ACT GC-3’
	Reverse	5’-TC CTT CTG GGT AGA CCT CTG GGA G-3’
Mouse XBP1	Forward	5’-A AAC AG A GTA GCA GCG CAG ACT GC-3’
	Reverse	5’-TC CTT CTG GGT AGA CCT CTG GG

XBP1: X-box binding protein 1 splicing.