Human urokinase-type plasminogen activator gene-modified bone marrow-derived mesenchymal stem cells attenuate liver fibrosis in rats by down-regulating the Wnt signaling pathway

Table 1 Primer sequences for polymerase chain reaction

Gene name	Direction	Sequence (5’-3’)
β-catenin	Forward	ACGGCAATCAGGAAAGCAA
	Reverse	ACAGACAGCACCTTCAGCACTC
Wnt4	Forward	GCCATCTCTTCAGCAGGTGTG
	Reverse	CATAGGCGATGTTGTCCGAGC
Wnt5a	Forward	ACTTGCACAACAATGAAGCAGGTC
	Reverse	CATAGGCGATGTTGTCCGAGC
uPA	Forward	CGAAGACTTCAGCGACGAAAC
	Reverse	CGAAGACTTCAGCGACGAAAC
GAPDH	Forward	TATGACTCTACCCACG
	Reverse	ATACTCAGCACCAGCATCACC

Table 2 Comparison of functional liver levels and liver fibrosis parameters in serum

Group	n	ALT (U/L)	AST (U/L)	TBIL (μmol/L)	ALB (g/L)	HA (μg/L)	LN (μg/L)	PCIII (μg/L)
uPA-BMSCs	10	74.95 ± 12.66bc	173.68 ± 21.10bc	18.66 ± 2.52bc	30.63 ± 4.25bc	105.71 ± 15.21bc	143.82 ± 18.99bc	27.14 ± 5.93bc
BMSCs	10	96.34 ± 14.97b	228.65 ± 25.66b	23.95 ± 5.09b	26.65 ± 3.79b	127.24 ± 17.79b	173.15 ± 22.32b	53.38 ± 6.31b
Model	10	126.76 ± 19.29a	287.67 ± 26.17a	32.13 ± 7.11a	22.60 ± 4.35a	187.66 ± 22.88a	224.45 ± 23.40a	73.89 ± 10.76a
Control	10	31.45 ± 7.75	67.51 ± 11.86	13.39 ± 3.23	35.95 ± 5.02	47.15 ± 6.58	97.23 ± 17.59	16.13 ± 3.13

^aP < 0.05 vs control group;

^bP < 0.05 vs model group;

^cP < 0.05 vs BMSCs group. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; TBIL: Total bilirubin; ALB: Albumin; HA: Hyaluronic acid; LN: Laminin; PCIII: Procollagen type III.