Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Dec 21, 2016; 22(47): 10364-10370
Published online Dec 21, 2016. doi: 10.3748/wjg.v22.i47.10364
Table 1 Association of ANGPTL2 expression with pathological features
GroupnGray value of ANGPTL2T valueP value
Tumor differentiationHigh-medium differentiation170.500 ± 0.221-4.7700
Low differentiation430.821 ± 0.240
T stageT1 + T2220.402 ± 0.198-5.9070
T3 + T4380.803 ± 0.280
Lymph node metastasisMetastatic350.680 ± 0.2105.5910
Non-metastatic250.404 ± 0.153
Table 2 Comparison of the gray value of ANGPTL2 protein in blank control, Lv-NC and Lv-ANGPTL2 PAMC82 gastric cancer cells
GroupANGPTL2/β-actin
Blank control0.53 ± 0.02
Lv-NC0.55 ± 0.02
Lv-ANGPTL20.90 ± 0.03a
Table 3 Comparison of the gray value of ANGPTL2 protein in blank control, Lv-NC-shRNA and Lv-ANGPTL2-shRNA PAMC82 gastric cancer cells
GroupANGPTL2/β-actin
Blank control0.99 ± 0.04
Lv-NC-shRNA0.95 ± 0.02
Lv-ANGPTL2-shRNA0.49 ± 0.03a
Table 4 Stable overexpression of ANGPTL2 contributed to the invasive ability of PAMC82 gastric cancer cells
GroupOD
Blank control0.279 ± 0.020
Lv-NC0.281 ± 0.019
Lv-ANGPTL20.451 ± 0.008b
Table 5 Knockdown of ANGPTL2 weakened the invasive ability of PAMC82 gastric cancer cells
GroupOD
Blank control0.301 ± 0.021
Lv-NC-shRNA0.299 ± 0.013
Lv-ANGPTL2-shRNA0.156 ± 0.018b