Therapeutics Advances
Copyright ©The Author(s) 2016.
World J Gastroenterol. Oct 28, 2016; 22(40): 8862-8868
Published online Oct 28, 2016. doi: 10.3748/wjg.v22.i40.8862
Table 1 Non-viral gene delivery systems toward the liver
MethodFunctional component
LipidsCationic lipids
PolymersCationic polymers
ProteinsNatural or chemically modified proteins in cationic nature
PeptidesLysine or arginine residues in peptides
Needle injectionMechanic force
Gene gunPressure
ElectroporationElectric pulse
Hydrodynamic deliveryHydrodynamic pressure
Table 2 Summary of the applications of hydrodynamic delivery for functional analysis of therapeutic genes related to the diseases of gastroenterology and hepatology
DiseaseTherapeutic GenesRef.
Nonalcoholic steatohepatitisInducible nitric oxcide synthetase[8]
HepatitisHBV knockdown[5,9]
HCV knockdown
Fulminant hepatitisNKG2D knockdown[7,10]
osteopontin knockdown
Liver injuryc-met[7,11]
caspase knockdown
Liver fibrosisplatelet-derived growth factor receptor beta knockdown[12]
Liver Regenerationfibroblast growth factor 7[13]
Fabry diseasealpha-galactosidase A[7]
Pancreatitispancreatitis associated protein 1[14]
Colon cancerIL-15[15]