miR-422a is an independent prognostic factor and functions as a potential tumor suppressor in colorectal cancer

Table 1 Relationship between miR-422a and clinical parameters in colorectal cancer tissues

Clinical parameter	Number of cases (n = 102)	miR-422a expression, median (min-max)	P value
Gender			0.704
Male	63	1.446 (0.074-130.774)
Female	39	1.617 (0.167-33.543)
Age (yr)			0.328
≤ median	49	1.637 (0.088-130.774)
> median	53	1.715 (0.074-21.871)
Tumor location			0.092
Colon	58	1.798 (0.088-130.774)
Rectum	44	1.887 (0.074-33.543)
Differentiation			0.150
Well	12	1.798 (0.074-130.744)
Moderate	67	1.922 (0.166-21.871)
Poor	23	2.088 (0.088-20.406)
Tumor size			0.527
≤ 5 cm	64	2.127 (0.074-130.744)
> 5 cm	38	2.353 (0.167-21.871)
Local invasion			0.004
T1–T2	55	3.257 (0.206-130.744)
T3–T4	47	1.617 (0.074-20.406)
Lymph node metastasis			0.002
No	70	2.863 (0.359-130.744)
Yes	32	1.445 (0.074-20.406)
TNM stage			< 0.001
I	23	3.877 (0.265-15.999)
II	42	3.253 (0.074-13.566)
III	37	1.445 (0.329-130.744)

Values are median (IQR: Inter-quartile range). TNM: Tumor node metastasis.

Table 2 Univariate and multivariate analyses of overall survival in colorectal cancer patients

Variable	Category	Univariate analysis			Multivariate analysis
		HR	95%CI	P value	HR	95%CI	P value
Gender	Male vs Female	0.943	0.493-1.872	0.810
Age	≤ Median vs > Median	1.436	0.528-3.157	0.148
Tumor location	Colon vs Rectum	0.862	0.484-1.706	0.603
Tumor size	≤ 5 cm vs > 5 cm	1.093	0.568-2.194	0.873
Differentiation	Well and moderate vs Poor	1.018	0.462-2.180	0.160	0.986	0.486-2.006	0.074
Local invasion	T1-T2 vs T3-T4	1.682	1.020-2.628	0.011	1.460	0.746-2.632	0.482
Lymph node metastasis	Yes vs No	1.182	0.634-2.530	< 0.0011	1.262	0.584-2.680	0.306
TNM stage	I and II vs III	2.738	1.509-4.265	< 0.0011	2.942	1.426-4.378	0.0031
MiR-422a level	Low vs High	0.306	0.108-0.763	0.0011	0.568	0.245-1.082	0.0151

¹There was a significant difference between the two groups (P < 0.05).