Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Mar 28, 2016; 22(12): 3341-3354
Published online Mar 28, 2016. doi: 10.3748/wjg.v22.i12.3341
Table 1 Clinicopathological data of the hepatocellular carcinoma cohort
Clinicopathologic parametersFrequency%
All cases30
Gender
Male2376.7
Female723.3
Age (yr)
< 50516.7
> 502583.3
Tumor size (cm)
< 5826.7
> 52273.3
Serum HBsAg
Positive2480.0
Negative620.0
Serum AFP (ng/mL)
< 25413.3
> 252686.7
Cirrhosis
Presence2790.0
Absence310.0
UICC stage
I + II1343.3
III + IV1756.7
Metastasis/Recurrence
Yes1860.0
No1240.0
Edmondson grade
Low (I/II)1033.3
High (III/IV)2066.7
Table 2 Primer sequences used for the amplification of different genes by quantitative PCR
GeneForward primer sequenceReverse primer sequence
AQP35'-CACAGCCGGCATCTTTGCTA-3'5'-TGGCCAGCACACACACGATA-3'
AQP95'-CTTAACAATTCACAAGGCACTT-3'5'-TCTCAGCCAGCTACTGATCTTC-3'
AFP5'-ACCCTGGTGTTGGCCAGTGC-3'5'-GCAGCGCTACACCCTGAGCT-3'
β-actin5'-TCACCCACACTGTGCCCATCTACGA-3'5'-CAGCGGAACCGCTCATTGCCAATGG-3'
Table 3 Correlation of AQP3 and AQP9 expression levels (low and high) to clinico-pathological data
FactorsAQP3
OR (95%CI)P valueAQP9
OR (95%CI)P value
LowHighLowHigh
Gender
Male4190.526 (0.074-3.748)0.6031617.111 (0.686-73.76)0.138
Female2594
Age (yr)
< 50141.000 (0.091-11.03)1.000320.205 (0.024-1.771)0.183
> 50520223
Tumor size (cm)
< 5353.800 (0.580-24.90)0.300620.474 (0.063-3.540)0.589
> 5319193
Serum HBsAg
Positive5191.316 (0.124-13.98)1.0002131.185 (0.166-8.475)1.000
Negative1542
Serum AFP (ng/mL)
< 253211.00 (1.271-95.23)0.041402.302 (0.107-49.54)1.000
> 25322215
Cirrhosis
Presence6212.116 (0.096-46.56)1.0001126.000 (0.780-46.17)0.102
Absence03143
UICC stage
I + II4410.00 (1.341-74.55)0.029240.022 (0.002-0.300)0.003
III + IV220231
Metastasis/recurrence
Yes1170.082 (0.008-0.839)0.02617112.67 (1.177-136.4)0.031
No5784
Edmondson grade
Low (I/II)3314.00 (1.741-112.6)0.016740.097 (0.009-1.029)0.047
High (III/IV)321181
Table 4 Cox regression analysis of patients with hepatocellular carcinoma
VariablesUnivariate
P value
HR95%CI
AQP3 expression (1 = low, 2 = high)4.9481.037-23.6050.045
AQP9 expression (1 = low, 2 = high)10.8351.488-78.8770.019
Gender (1 = Male, 2 = Female)2.6810.852-8.4360.092
Age (1 < 50, 2 ≥ 50)2.5790.332-20.0490.365
Tumor size (1 < 5 cm, 2 ≥ 5)1.2610.334-4.7590.732
Serum HBsAg (1 = Positive, 2= Negative)0.5750.153-2.1540.411
Serum AFP (1 < 25 ng/mL, 2 ≥ 25 ng/mL)1.1020.293-4.1460.886
Cirrhosis (1 = Presence, 2 = Absence)1.4010.373-5.2680.618
UICC stage (1 = I + II, 2 = III + IV)0.1150.027-0.4850.003
Metastasis/Recurrence (1 = Yes, 2 = No)0.2280.065-0.8040.021
Edmondson grade [1 = Low (I/II), 2 = High (III/IV)]0.5600.175-1.7910.328