Isolation and diagnosis of Helicobacter pylori by a new method: Microcapillary culture

Table 1 Helicobacter pylori isolation results of 26 biopsies tested according to different diagnostic methods

Biopsies	CC	MCM	HE	CLO
1	+	+	+	+
2	+	+	-	-
3	-	+	+	+
4	-	+	+	+
5	+	+	-	-
6	+	+	+	+
7	-	+	+	+
8	-	+	+	+
9	+	+	-	-
10	-	+	+	+
11	+	+	-	-
12	-	+	+	+
13	+	+	+	+
14	+	+	+	+
15	-	+	+	+
16	-	+	+	+
17	+	+	+	+
18	+	+	-	-
19	-	+	+	+
20	+	+	-	-
21	-	+	+	+
22	+	+	-	-
23	+	+	-	-
24	-	+	+	+
25	+	+	+	+
26	-	-	+	+

CC: Classical culture; HE: Histopathologic examination; CLO: Rapid urease test; MCM: Microcapillary culture method.

Table 2 Diagnostic performance parameters of the microcapillary culture method, classical culture, histopathologic examination and rapid urease test

	H. pylori (+) reference	H. pylori (-) control
MCM1
+	25	5
-	1	21
Total	26	26
CC2
+	14	-
-	12	26
Total	26	26
HE3 and CLO4 test
+	17	-
-	9	26
Total	26	26

¹Diagnostic performance parameters of microcapillary culture method (MCM): Sensitivity: 96%, specificity: 80%, positive predictive value (PPV): 83%, negative predictive value (NPV): 95%, kappa: 0.76;

²Diagnostic performance parameters of classical culture (CC): Sensitivity: 54%, specificity: 100%, PPV: 100%, NPV: 68%, kappa: 0.54;

³Diagnostic performance parameters of histopathologic examination (HE): Sensitivity: 65%, specificity: 100%, PPV: 100%, NPV: 74%, kappa: 0.64;

⁴Diagnostic performance parameters of rapid urease (CLO) test: Sensitivity: 65%, specificity: 100%, PPV: 100%, NPV: 74%, kappa: 0.64. H. pylori: Helicobacter pylori.