Basic Study
Copyright ©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 7, 2015; 21(9): 2622-2628
Published online Mar 7, 2015. doi: 10.3748/wjg.v21.i9.2622
Isolation and diagnosis of Helicobacter pylori by a new method: Microcapillary culture
Adil M Allahverdiyev, Melahat Bagirova, Reyhan Caliskan, Hrisi Bahar Tokman, Hayat Aliyeva, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Pelin Yuksel, Fatma Kalayci, Mustafa Aslan, Yusuf Erzin, Kadir Bal, Bekir S Kocazeybek
Adil M Allahverdiyev, Melahat Bagirova, Gokce Unal, Olga Nehir Oztel, Emrah Sefik Abamor, Hilal Toptas, Department of Bioengineering, Yildiz Technical University, 34201 Istanbul, Turkey
Reyhan Caliskan, Hrisi Bahar Tokman, Pelin Yuksel, Fatma Kalayci, Mustafa Aslan, Bekir S Kocazeybek, Department of Medical Microbiology, Cerrahpasa Faculty of Medicine, Istanbul University, 34098 Istanbul, Turkey
Hayat Aliyeva, Department of Microbiology and Immunology, Azerbaijan Medical University, AZ1022 Baku, Azerbaijan
Yusuf Erzin, Kadir Bal, Department of Gastroenterology, Cerrahpasa Faculty of Medicine, Istanbul University, 34098 Istanbul, Turkey
Author contributions: Allahverdiyev AM designed and drafted the research; Tokman HB and Kocazeybek BS contributed to design of the research, assisted in the scientific writing of the paper; Erzin Y and Bal K provided the biopsy samples; Caliskan R, Aliyeva H, Unal G, Oztel ON, Abamor ES, Toptas H and Kalayci F performed all the microbiological procedures; Bagirova M, Yuksel P and Aslan M interpreted the results and reviewed the literature.
Supported by Yildiz Technical University Scientific Research Projects Coordinatorship, No. 2012-07-04-KAP09.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Adil M Allahverdiyev, Professor, Department of Bioengineering, Yildiz Technical University, Yildiz Mh, 34201 Istanbul, Turkey. adilmoglu@gmail.com
Telephone: +90-212-3834639 Fax: +90-212-3834625
Received: April 14, 2014
Peer-review started: April 14, 2014
First decision: June 10, 2014
Revised: July 26, 2014
Accepted: November 8, 2014
Article in press: November 11, 2014
Published online: March 7, 2015
Processing time: 329 Days and 11.1 Hours
Core Tip

Core tip: Nowadays, isolation of Helicobacter pylori (H. pylori) from gastric tissues by using classical culture is accepted as the gold standard, according to Maastricht criteria. Additionally, surveillance of increased or decreased minimal inhibitory concentration values of antimicrobial substances, according to applied treatment policy and geographical regions, may only be possible with the cultivation of bacteria. On the other hand, the diagnostic sensitivity of classical culture is low due to problems in standardization of the medium in order to provide microaerophylic conditions. In this study, for the first time, we report the high diagnostic sensitivity of the microcapillary culture method for diagnosis of H. pylori.