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©The Author(s) 2015.
World J Gastroenterol. Dec 21, 2015; 21(47): 13360-13367
Published online Dec 21, 2015. doi: 10.3748/wjg.v21.i47.13360
Published online Dec 21, 2015. doi: 10.3748/wjg.v21.i47.13360
Table 1 Sensitivity of nested polymerase chain reaction assay for the amplification of the 572-bp HBV X antigen fragment n (%)
| No. of samples (n = 198) | Sensitivity | ||
| HCC (n =121) | Carrier (n =77) | ||
| PCR amplification | 46 (38.0) | 23 (29.9) | 34.8% |
Table 2 Evaluation of Fok-I nested polymorphism analysis assay for the detection of V5M mutations from 69 samples amplified by nested PCR by comparing with the results of the direct sequencing method n (%)
| Type of HBxAg 5th codon | Result for | |||
| Fok-I nested PRA | Direct sequencing | |||
| HCC(n =46) | Carrier(n =23) | HCC(n =46) | Carrier(n =23) | |
| Wild type | 24 (52.2) | 23 (100) | 24 (52.2) | 23 (100) |
| V5M | 17 (37.0) | 0 (0) | 17 (37.0) | 0 (0) |
| Wild type + V5M | 5 (10.8) | 0 (0) | 5 (10.8) | 0 (0) |
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Citation: Kim H, Hong SH, Lee SA, Gong JR, Kim BJ. Development of
Fok -I based nested polymerase chain reaction-restriction fragment length polymorphism analysis for detection of hepatitis B virus X region V5M mutation. World J Gastroenterol 2015; 21(47): 13360-13367 - URL: https://www.wjgnet.com/1007-9327/full/v21/i47/13360.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i47.13360