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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 21, 2015; 21(47): 13360-13367
Published online Dec 21, 2015. doi: 10.3748/wjg.v21.i47.13360
Published online Dec 21, 2015. doi: 10.3748/wjg.v21.i47.13360
Development of Fok-I based nested polymerase chain reaction-restriction fragment length polymorphism analysis for detection of hepatitis B virus X region V5M mutation
Hong Kim, Seok-Hyun Hong, Seoung-Ae Lee, Jeong-Ryeol Gong, Bum-Joon Kim, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South Korea
Author contributions: Kim H and Hong SH contributed equally to this work; Kim BJ was the guarantor and designed the study; Kim H and Hong SH participated in the acquisition, analysis, and interpretation of the data, and drafted the initial manuscript; Lee SA, Gong JR revised the article critically for important intellectual content.
Supported by a National Research Foundation (NRF) of Korea grant funded by the Korean government (Ministry of Education, Science, and Technology, MEST), Grant No. 2013-005810.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board of Seoul National University Hospital (Seoul) (IRB No. 1404-070-572).
Informed consent statement: All study participants, or their legal guardian, provided informed written consent prior to study enrollment.
Conflict-of-interest statement: There are no conflicts of interest to report.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Bum-Joon Kim, Professor, Department of Biomedical Sciences, Microbiology and Immunology, and Liver Research Institute, Seoul National University College of Medicine, Seoul 110-799, South Korea. kbumjoon@snu.ac.kr
Telephone: +82-2-7408316 Fax: +82-2-7430881
Received: June 8, 2015
Peer-review started: June 11, 2015
First decision: July 10, 2015
Revised: July 17, 2015
Accepted: September 30, 2015
Article in press: September 30, 2015
Published online: December 21, 2015
Peer-review started: June 11, 2015
First decision: July 10, 2015
Revised: July 17, 2015
Accepted: September 30, 2015
Article in press: September 30, 2015
Published online: December 21, 2015
Core Tip
Core tip: In the present study, we developed a reliable and cost-effective Fok-I nested polymerase chain reaction-restriction fragment length polymorphism analysis (PRA) method for the detection of V5M from chronic patients with genotype C2 infection. In addition, our epidemiological data based on the Fok-I nested PRA method strongly support the previous reports that V5M may play a very pivotal role in hepatocarcinogenesis, at least in chronic patients infected with genotype C2.