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©The Author(s) 2015.
World J Gastroenterol. Oct 21, 2015; 21(39): 11141-11151
Published online Oct 21, 2015. doi: 10.3748/wjg.v21.i39.11141
Published online Oct 21, 2015. doi: 10.3748/wjg.v21.i39.11141
Table 1 The primers and probes used for the low density lipoprotein receptor genes
exon 8 (c.1171G > A) | F5'-CTACAAGTGCCAGTGTGAGGAA-3' | |
R5’_CCCACCACTCTGCTTGTAAGGCGTGAGGCCGCC-3' | ||
Allele 1 specific probe VIC-ACACGAAGGCCTGC-NFQ | ||
LDLR primers | Allele 2 specific probe 6-FAM-ACACGAAGACCT GC-NFQ | |
exon 10 (c.1413G > A) | F5'- CGGCGTCTCTTCCTATGACA-3' | |
R5'- GTCCAGTAGATGTTGCTGTGGAT-3' | ||
Allele 1 specific probe VIC-ATCAGCAGGGAC ATC-NFQ | ||
Allele 2 specific probe 6-FAM-TCAGCAGAGACA TC-NFQ |
Table 2 The frequency of the genotypes of gene polymorphisms in the studied groups n (%)
Non responders (n = 301) | Responders (n = 356) | Control(n = 160) | P value | |
LDR exon8 c.1171G>A | < 0.001 | |||
AA | 157 (52.2) | 13 (3.6) | 6 (3.8) | |
GA | 92 (30.6) | 54 (15.2) | 85 (53.1) | |
GG | 52 (17.2) | 289 (81.2) | 69 (43.1) | |
LDR exon10 c.1413G>A | 0.204 | |||
AA | 48 (15.9) | 51 (14.3) | 15 (9.4) | |
GA | 191 (63.5) | 215 (60.4) | 109 (68.1) | |
GG | 62 (20.6) | 90 (25.3) | 36 (22.5) |
Table 3 Allele frequency and risk ratio in non-responders vs responders
Non responders | Responders | P value | OR (95%CI) | |
n (%) | n (%) | |||
LDR exon 8 c.1171G>Aallele | ||||
A | 406 (67.4) | 80 (11.2) | < 0.001 | 16.4 (12.3-21.8) |
G | 196 (32.6) | 632 (88.8) | 1 | |
exon 10 c.1413 G>Aallele | ||||
A | 287 (47.7) | 317 (44.5) | 0.253 | 1.13 (0.9-1.4) |
G | 315 (52.3) | 395 (55.5) | 1 |
Table 4 Steatosis and body mass index mean level in different LDLR genotypes in hepatitis C virus patients n (%)
LDR exon 8 c.1171 G>A | P value | LDR exon 10 c.1413 G>A | P value | |||||
AA | GA | GG | AA | GA | GG | |||
BMI (kg/m2) | 28.7 ± 4.7 | 28.1 ± 4.8 | 26.6 ± 4.3 | < 0.001 | 27.3 ± 4.3 | 27.5 ± 4.8 | 27.4 ± 4.4 | 0.887 |
Steatosis | ||||||||
0 | 64 (37.6) | 70 (47.9) | 204 (59.8) | < 0.001 | 50 (50.5) | 208 (51.2) | 80 (52.6) | 0.938 |
1,2,3 | 106 (62.4) | 76 (52.1) | 137 (40.2) | 49 (49.5) | 198 (48.8) | 72 (47.4) |
- Citation: Naga M, Amin M, Algendy D, Elbadry A, Fawzi M, Foda A, Esmat S, Sabry D, Rashed L, Gabal S, Kamal M. Low-density lipoprotein receptor genetic polymorphism in chronic hepatitis C virus Egyptian patients affects treatment response. World J Gastroenterol 2015; 21(39): 11141-11151
- URL: https://www.wjgnet.com/1007-9327/full/v21/i39/11141.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i39.11141