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©The Author(s) 2015.
World J Gastroenterol. Oct 21, 2015; 21(39): 11118-11126
Published online Oct 21, 2015. doi: 10.3748/wjg.v21.i39.11118
Published online Oct 21, 2015. doi: 10.3748/wjg.v21.i39.11118
Table 1 Primers, GenBank accession numbers, and amplicon size of genes for reverse transcription-polymerase chain reaction
| Gene | Primer | Accession Number | Amplicon size (bp) |
| FOXA2 | TGTCAGGAGCACAAGCGAGG (F) | NM-012743.1 | 194 bp |
| GGGTGGTTGAAGGCGTAATGGTG (R) | |||
| SOX17 | GGCACGGAACCCAACCAGC (F) | NM-001107902.1 | 119 bp |
| CAGTCGTGTCCCTGGTAGGGAAGAC (R) | |||
| AFP | TCTGAAACGCCATCGAAATGCC (F) | NM-012493.2 | 285 bp |
| AATGTAAATGTCGGCCAGTCCCT (R) | |||
| ALB | GGCACAGTGCTTGCAGAATTTCAG (F) | NM-134326.2 | 272 bp |
| CACAGACGGTTCAGGATGGCAG (R) | |||
| CK8 | TGAAGGATGCCAATGCCAAGCTG (F) | NM-199390.1 | 237 bp |
| AACTCAGTCCTCCTGCGTAGCC (R) | |||
| CK18 | ACGATTTCAGTCTCAACGACGCC (F) | NM-053976.1 | 147 bp |
| TCCCTTCTTCTGAGCCTTAGTGCC (R) | |||
| CK19 | GCTCAGCATGAAAGCTGCCCT (F) | NM-199498.1 | 299 bp |
| CGCACTGGTAGCAAGGTAGGAG (R) | |||
| HNF4α | GATGTGCTGCTCCTAGGCAATGAC (F) | NM-001270931.1 | 261 bp |
| CTGCCGGTCGTTGATGTAATCCTC (R) | |||
| GADPH | CCTTCATTGACCTCAACTAC (F) | NM-017008.4 | 594 bp |
| GGAAGGCCATGCCAGTGAGC (R) |
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Citation: Sun C, Hu JJ, Pan Q, Cao Y, Fan JG, Li GM. Hepatic differentiation of rat induced pluripotent stem cells
in vitro . World J Gastroenterol 2015; 21(39): 11118-11126 - URL: https://www.wjgnet.com/1007-9327/full/v21/i39/11118.htm
- DOI: https://dx.doi.org/10.3748/wjg.v21.i39.11118