Neurochemical features of endomorphin-2-containing neurons in the submucosal plexus of the rat colon

Table 1 Percentages of submucosal neurons with the co-localization of endomorphin-2 and various markers

Marker pair	Submucosal ganglion neurons (n1 and n2, mean ± SD)	Double-labeled neurons (n, mean ± SD)	Percentage (%) (mean ± SE)
CGRP/EM-2	1.58 ± 1.4 and 5.0 ± 1.1	0	CGRP of EM-2 (n/n2) 0
ChAT/EM-2	6.0 ± 2.4 and 3.5 ± 1.6	3.2 ± 1.2	ChAT of EM-2 (n/n2) 91 ± 2.6
			EM-2 of ChAT (n/n1) 53 ± 2.8
NOS/EM-2	2.3 ± 1.1 and 5.2 ± 1.4	2.3 ± 1.1	NOS of EM-2 (n/n2) 44 ± 4.7
			EM-2 of NOS (n/n1) 100.0
NSE/EM-2	6.2 ± 3.7 and 4.2 ± 2.9	4.2 ± 2.9	NSE of EM-2 (n/n2) 100.0
			EM-2 of NSE (n/n1) 68 ± 1.5
SP/EM-2	2.4 ± 1.0 and 5.0 ± 1.2	1.8 ± 0.6	SP of EM-2 (n/n2) 36 ± 2.4
			EM-2 of SP (n/n1) 75 ± 3.4
VIP/EM-2	1.5 ± 0.5 and 5.0 ± 1.3	1.5 ± 0.5	VIP of EM-2 (n/n2) 30 ± 2.5
			EM-2 of VIP (n/n1) 100.0

VIP: Vasoactive intestinal peptide; EM-2: Endomorphin-2; SP: Substance P; NSE: Neuron-specific enolase; NOS: Nitric oxide synthetase; CGRP: Calcitonin gene-related peptide; ChAT: Choline acetyltransferase.