MiR-9a-5p regulates proliferation and migration of hepatic stellate cells under pressure through inhibition of Sirt1

Table 1 Primers used in real-time polymerase chain reaction

Gene	Forward primer (5’-3’)	Reverse primer (5’-3’)	Product size (bp)
α-SMA	GCCACTGCTGCTTCCTCTTCTT	CCGCCGACTCCATTCCAATGAA	136
Col1	GCTCGTGGATTGCCTGGAACAG	CACCGACAGCACCATCGTTACC	235
Sirt1	TGTGGGTCTATGCCTTTCTGGGT	GCTTGCGTGTGATGCTCTGTC	239
GAPDH	ACGGCAAGTTCAACGGCACAG	CCACGACATACTCAGCACCAGC	132

Table 2 MiRNAs differentially expressed in response to pressure overload

miRNA	Log2FC	Fold change	FDR
Upregulated microRNAs
rno-miR-206-3p	3.347	10.176	2.22E-05
rno-miR-210-3p	1.903	3.740	9.61E-05
rno-miR-494-5p	1.691	3.229	0.001401
rno-miR-345-3p	3.127	8.736	0.001984
rno-miR-592	2.990	7.945	0.002623
rno-miR-124-3p	1.656	3.151	0.002647
rno-miR-429	1.862	3.635	0.003247
rno-miR-34c-5p	2.612	6.113	0.007838
rno-miR-34b-5p	2.482	5.587	0.008211
rno-miR-34a-3p	2.434	5.404	0.008598
rno-miR-450a-3p	2.309	4.955	0.008823
rno-miR-199a-5p	2.091	4.260	0.009277
rno-miR-222-3p	2.608	6.097	0.009440
rno-miR-450a-5p	2.373	5.180	0.009558
rno-miR-34a-5p	2.319	4.990	0.009845
rno-miR-494-3p	1.671	3.184	0.019836
rno-miR-377-5p	1.877	3.673	0.037294
rno-miR-9a-5p	2.124	4.359	1.23E-05
Downregulated miRNAs
rno-miR-26a-3p	-3.412	0.094	1.54E-04
rno-miR-222-5p	-2.740	0.150	0.007077
rno-miR-335	-2.428	0.186	0.009698
rno-miR-194-3p	-2.785	0.145	0.021509
rno-miR-103-1-5p	-2.555	0.170	0.028431
rno-miR-664-1-5p	-1.972	0.255	0.041855
rno-miR-188-3p	-2.472	0.180	0.043457