Reciprocal impact of host factors and Helicobacter pylori genotypes on gastric diseases

Table 1 Oligonucleotide primers used for polymerase chain reaction

Gene	Primer	Sequence (5'→3')	PCR products (bp)	Annealing temperature (°C)	Ref.
16S rRNA	HP1	GCAATCAGCGTCAGTAATGTT C	519	58.5	[85]
	HP2	GCTAAGAGATCAGCCTATGTCC
vacA (s1, s2)	VA1F	ATGGAAATACAAGAAACACACC	s1: 259	56.0	[25]
	VA1R	CTGCTTGAATGCGCCAAACTTTAATC	s2: 286
vacA (m1, m2)	VAG-F	CAATCTGTCCAATCAAGCGAG	m1: 570	58.5	[25]
	VAG-R	GCGTCAAAATAATTCCAAGG	m2: 645
cagA	D008	ATAATGCTAAATTAGACAACTTGAGCGA	298	58.5	[86]
	R008	TTAGAATAATCAACAAACATCACGCCAT

PCR: Polymerase chain reaction.

Table 2 Distribution of genotypes in Helicobacter pylori isolates n (%)

Characteristic		CG	GU	DU	Total
No. of cases		113 (48.5)	64 (27.5)	56 (24.0)	233 (100)
Sex	Female	62 (54.9)	27 (42.2)	15 (26.8)	104 (44.6)
	Male	51 (45.1)	37 (57.8)	41 (73.2)	129 (55.4)
Age	< 40 yr	43 (38.1)	5 (7.8)	17 (30.4)	65 (27.9)
	> 40 yr	70 (61.9)	59 (92.2)	39 (69.6)	168 (72.1)
vacA	s1	83 (73.5)	53 (82.8)	55 (98.2)	191 (82.0)
	s2	30 (26.5)	11 (17.2)	1 (1.8)	42 (18.0)
	m1	32 (28.3)	16 (25)	20 (35.7)	68 (29.2)
	m2	81 (71.7)	48 (75)	34 (60.7)	163 (70.0)
	s1m1	24 (21.2)	15 (23.4)	19 (33.9)	58 (24.9)
	s1m2	59 (52.2)	38 (59.4)	34 (60.7)	131 (56.2)
	s2m1	6 (5.3)	1 (1.6)	0	7 (3.0)
	s2m2	24 (21.2)	10 (15.6)	1 (1.8)	35 (15.0)
cagA	+	82 (72.6)	50 (78.1)	37 (66.1)	169 (72.5)
	-	31 (27.4)	14 (21.9)	19 (33.9)	64 (27.5)
s1m1cagA	+	17 (15.0)	10 (15.6)	10 (17.9)	37 (15.9)
	-	7 (6.2)	5 (7.8)	9 (16.1)	21 (9.0)
s1m2cagA	+	41 (36.3)	30 (46.9)	24 (42.9)	95 (40.8)
	-	18 (15.9)	8 (12.5)	10 (17.9)	36 (15.5)
s2m1cagA	+	5 (4.4)	1 (1.6)	0	6 (2.6)
	-	1 (0.9)	0	0	1 (0.4)
s2m2cagA	+	19 (16.8)	9 (14.1)	1 (1.8)	29 (12.4)
	-	5 (4.4)	1 (1.6)	0	6 (2.6)
s1m1m2cagA	+	0	0	2 (3.6)	2 (0.8)
	-	0	0	0	0

CG: Chronic gastritis; DU: Duodenal ulcer; GU: Gastric ulcer.