Insulin-like growth factor-1 mRNA isoforms and insulin-like growth factor-1 receptor mRNA expression in chronic hepatitis C

Table 1 Quantitative expression of insulin-like growth factor-1 mRNA isoforms, total mRNA insulin-like growth factor-1, and insulin-like growth factor-1R mRNA (mean ± SD) normalized in relation to housekeeping genes in chronic hepatitis C and in control organ

mRNA	Control(n = 7)	CH-C(n = 34)	P value	CH-C		P value
				Women (n = 16)	Men (n = 18)
class 1 (P1)	0.19 ± 0.21	0.45 ± 0.45	P = 0.025	0.63 ± 0.59	0.28 ± 0.17	P = 0.003
class 2 (P2)	0.08 ± 0.07	0.15 ± 0.17	P = 0.164	0.20 ± 0.15	0.11 ± 0.18	P = 0.006
IGF-1A	1.30 ± 1.31	5.28 ± 9.94	P = 0.009	7.88 ± 13.83	2.97 ± 3.32	P = 0.027
IGF-1B	0.18 ± 0.18	0.28 ± 0.40	P = 0.486	0.35 ± 0.45	0.22 ± 0.35	P = 0.042
IGF-1C	0.04 ± 0.05	0.21 ± 0.57	P = 0.031	0.35 ± 0.82	0.08 ± 0.06	P = 0.046
Total IGF-1	1.52 ± 1.52	5.77 ± 10.85	P = 0.014	8.58 ± 15.09	3.26 ± 3.70	P = 0.027
IGF-1R	0.26 ± 0.18	0.14 ± 0.04	P = 0.016	0.14 ± 0.05	0.14 ± 0.04	P = 0.295

IGF-1: Insulin-like growth factor 1; CH-C: Chronic hepatitis C.

Table 2 Tissue expression of insulin-like growth factor-1 mRNA isoforms and insulin-like growth factor-1R mRNA (mean ± SD) as related to grading and staging in hepatitis C virus-infected livers

mRNA	Grading1 1 (n = 15)	Grading 2 (n = 16)	P value	Staging1 1 (n = 21)	Staging 2 (n = 8)	P value
class 1 (P1)	0.63 ± 0.61	0.33 ± 0.20	P = 0.037	0.50 ± 0.55	0.41 ± 0.23	P = 0.905
class 2 (P2)	0.25 ± 0.22	0.09 ± 0.06	P = 0.009	0.19 ± 0.20	0.11 ± 0.07	P = 0.549
IGF-1A	8.67 ± 14.34	2.42 ± 1.45	P = 0.008	6.55 ± 12.47	3.01 ± 1.77	P = 0.943
IGF-1B	0.44 ± 0.55	0.15 ± 0.10	P = 0.012	0.34 ± 0.49	0.18 ± 0.12	P = 0.720
IGF-1C	0.36 ± 0.84	0.08 ± 0.06	P = 0.093	0.27 ± 0.72	0.09 ± 0.07	P = 0.582
Total IGF-1	9.47 ± 15.66	2.65 ± 1.58	P = 0.006	7.16 ± 13.61	3.29 ± 1.91	P = 0.830
IGF-1R	0.12 ± 0.04	0.16 ± 0.03	P = 0.025	0.14 ± 0.04	0.16 ± 0.04	P = 0.198

¹Parameters evaluated in a semi-quantitative scale (see Material and Methods). IGF-1: Insulin-like growth factor 1.

Table 3 Values of Spearman’s coefficient for correlation between expression of insulin-like growth factor-1, insulin-like growth factor-1R mRNAs, and clinical data in hepatitis C virus-infected patients

	Class 1 (P1)	Class 2 (P2)	IGF-1A	IGF-1B	IGF-1C	Total IGF-1	IGF-1R
Age (yr)	-0.5451	-0.6441	-0.5821	-0.6541	-0.5321	-0.5961	0.3721
BMI	-0.5271	-0.4521	-0.4681	-0.4141	-0.4891	-0.4681	0.060
Grading	-0.4311	-0.5691	-0.4461	-0.4301	-0.2851	-0.4571	0.4561
Staging	-0.223	-0.3461	-0.182	-0.187	-0.160	-0.190	0.264
Steatosis (%)	-0.4151	-0.5711	-0.4121	-0.4081	-0.4541	-0.4221	0.125
ALT (U/L)	-0.5381	-0.6161	-0.5401	-0.5771	-0.5331	-0.5501	0.181
AST (U/L)	-0.4341	-0.5691	-0.3781	-0.4001	-0.3421	-0.3921	0.212
AFP (ng/mL)	-0.291	-0.3901	-0.168	-0.271	-0.240	-0.180	0.311
HCV RNA (IU/mL)	-0.330	-0.3881	-0.3571	-0.244	-0.096	-0.3451	0.4031
Total protein (g/dL)	0.302	0.251	0.324	0.3751	0.3661	0.319	0.141
Albumins (g/dL)	0.293	0.3811	0.303	0.3491	0.222	0.295	0.123
Gamma globulins (g/dL)	-0.061	-0.164	0.039	0.045	0.174	0.044	0.138
Blood platelets (G/L)	0.273	0.3721	0.235	0.265	0.138	0.232	-0.099
Cholesterol (mg/dL)	0.3861	0.286	0.4091	0.4051	0.372	0.4041	0.048
GGTP (U/L)	-0.384	-0.6031	-0.284	-0.399	-0.367	-0.307	0.330
Blood glucose (mg/dL)	-0.083	-0.226	-0.190	-0.112	-0.084	-0.201	0.152
HOMA-IR	-0.067	-0.157	-0.237	-0.140	-0.184	-0.234	-0.019
Insulin (μU/mL)	-0.020	-0.082	-0.145	-0.079	-0.125	-0.135	-0.045

¹The numbers indicate values of r coefficient for which the P < 0.05. IGF-1: Insulin-like growth factor 1; HCV: Hepatitis C virus; AFP: Alpha fetoprotein.