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©The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 7, 2015; 21(13): 3867-3875
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3867
Published online Apr 7, 2015. doi: 10.3748/wjg.v21.i13.3867
Insulin-like growth factor-1 mRNA isoforms and insulin-like growth factor-1 receptor mRNA expression in chronic hepatitis C
Aldona Kasprzak, Wiesława Przybyszewska, Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland
Agnieszka Adamek, Iwona Mozer-Lisewska, Department of Infectious Diseases, Poznan University of Medical Sciences, 61-285 Poznan, Poland
Przemysław Pyda, Jacek Szmeja, Department of General Surgery, Gastroenterological Oncology and Plastic Surgery, Poznan University of Medical Sciences, 60-355 Poznan, Poland
Agnieszka Seraszek-Jaros, Department of Bioinformatics and Computational Biology, Chair of Clinical Pathomorphology, Poznan University of Medical Sciences, 60-529 Poznan, Poland
Agata Lanzafame, Anna Surdacka, Department and Clinics of Conservative Dentistry and Periodontology, Poznan University of Medical Sciences, 60-812, Poznan, Poland
Maria Koczorowska, Department of Molecular Virology, Adam Mickiewicz University, 61-614 Poznan, Poland
Author contributions: Kasprzak A and Adamek A contributed equally to this work; Kasprzak A and Adamek A designed the research; Kasprzak A, Adamek A, Przybyszewska W, Pyda P and Szmeja J performed the research; Kasprzak A, Adamek A, Seraszek-Jaros A, Lanzafame A, Surdacka A and Mozer-Lisewska I analyzed the data; Seraszek-Jaros A performed biostatistics; Koczorowska M contributed new reagents/analytic tools; Kasprzak A and Adamek A wrote the paper.
Supported by Minister of Education and Science, Warsaw, Poland, No. NN401009437.
Ethics approval: Committee on Bioethics, Poznan University of Medical Sciences, 61-701 Poznan, Poland (No. 22/09).
Institutional animal care and use committee: Not applicable.
Conflict-of-interest: All authors declare that they have no relevant or material financial interests that relate to the research described in this paper.
Data sharing: No additional data available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Aldona Kasprzak, MD, PhD, Professor, Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki Street, 60-781 Poznan, Poland. akasprza@ump.edu.pl
Telephone: +48-61-8546441 Fax: +48-61-8546440
Received: August 26, 2014
Peer-review started: August 27, 2014
First decision: September 15, 2014
Revised: October 11, 2014
Accepted: January 8, 2015
Article in press: January 8, 2015
Published online: April 7, 2015
Processing time: 224 Days and 6.2 Hours
Peer-review started: August 27, 2014
First decision: September 15, 2014
Revised: October 11, 2014
Accepted: January 8, 2015
Article in press: January 8, 2015
Published online: April 7, 2015
Processing time: 224 Days and 6.2 Hours
Core Tip
Core tip: Hepatitis C virus (HCV) may induce alteration of insulin-like growth factor (IGF)-1 splicing profile. A quantitative polymerase chain reaction analysis confirmed higher relative expression of total IGF-1 mRNA and of IGF-1 mRNAs isoforms P1, A, and C in HCV-infected livers as compared to the control. An increase in inflammatory activity (grading) of HCV-infected livers was linked to decreased IGF-1 mRNA expression, an altered profile of mRNA isoforms, and to an increase in IGF-1R mRNA expression. Decreased expression level of IGF-1 mRNA isoforms and an increased liver expression of IGF-1R mRNA were associated with indicators of liver damage (e.g., grading, staging, steatosis, and liver serum enzyme activity), and may be of prognostic significance.