Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration

Table 1 Gene ontology analysis of senescent hepatocyte gene expression signature

Gene list	Ontology	% list	P value	Fold enrichment
A: senescence induced	Steroid metabolism	8.5	5.50E-12	7.4
	Endoplasmic reticulum	15.7	7.10E-9	2.8
	Oxidation/reduction	12.1	8.00E-9	3.5
	Drug metabolism	4.8	7.80E-8	8.6
	Lysosome	6.0	2.20E-6	4.9
	Retinol metabolism	4.0	2.20E-6	8.3
	Signal peptide	27.8	1.40E-5	1.7
	Iron ion binding	6.5	3.20E-5	3.6
	Defence response	8.5	3.00E-4	2.5
	Lipid biosynthesis	5.6	5.40E-4	3.1
	Lipid catabolism	4.0	7.20E-4	4.1
	Secreted	14.9	1.50E-3	1.7
	Regulation of apoptosis	9.3	1.90E-3	2.0
	Inflammatory response	4.4	1.50E-2	2.4
B: secreted	Disulfide bond	62.2	3.90E-10	4.1
	Plasma	16.2	9.10E-7	32.6
	Glycoprotein	56.8	2.10E-5	2.5
	Defence response	21.6	2.10E-4	6.0
	Immune response	16.2	1.10E-2	4.2
	Lipid binding	13.5	1.40E-2	5.0
	Cell adhesion	13.5	5.80E-2	3.3
	Regulation of proliferation	13.5	8.30E-2	2.9

Gene ontology enrichment in (A) senescence-induced genes and (B) senescence-induced genes falling in the “Secreted” ontology was analysed using DAVID.