Irvine KM, Skoien R, Bokil NJ, Melino M, Thomas GP, Loo D, Gabrielli B, Hill MM, Sweet MJ, Clouston AD, Powell EE. Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration. World J Gastroenterol 2014; 20(47): 17851-17862 [PMID: 25548483 DOI: 10.3748/wjg.v20.i47.17851]
Corresponding Author of This Article
Elizabeth E Powell, Professor, Director, Centre for Liver Disease Research, School of Medicine, The University of Queensland, Translational Research Institute, Brisbane, 37 Kent St, Woolloongabba, Brisbane 4102, Australia. e.powell@uq.edu.au
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Dec 21, 2014; 20(47): 17851-17862 Published online Dec 21, 2014. doi: 10.3748/wjg.v20.i47.17851
Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration
Katharine M Irvine, Richard Skoien, Nilesh J Bokil, Michelle Melino, Gethin P Thomas, Dorothy Loo, Brian Gabrielli, Michelle M Hill, Matthew J Sweet, Andrew D Clouston, Elizabeth E Powell
Katharine M Irvine, Richard Skoien, Michelle Melino, Andrew D Clouston, Elizabeth E Powell, Centre for Liver Disease Research, School of Medicine, The University of Queensland, Translational Research Institute, Brisbane 4102, Australia
Nilesh J Bokil, Matthew J Sweet, Institute for Molecular Bioscience, The University of Queensland, Brisbane 4067, Australia
Gethin P Thomas, Dorothy Loo, Brian Gabrielli, Michelle M Hill, The University of Queensland Diamantina Institute, The University of Queensland, Translational Research Institute, Brisbane 4102, Australia
Elizabeth E Powell, Department of Gastroenterology and Hepatology, Princess Alexandra Hospital, Brisbane 4102, Australia
Author contributions: Irvine KM, Skoien R, Sweet MJ, Clouston AD and Powell EE designed the research; Irvine KM, Skoien R, Bokil NJ, Melino M, Thomas GP and Loo D performed the research; Sweet MJ, Gabrielli B and Hill MM contributed new reagents and tools; Irvine KM, Skoein R, Bokil NJ and Hill MM analysed the data; Irvine KM, Skoein R and Powell EE wrote the paper.
Supported by the National Health and Medical Research Council of Australia (NHMRC), APP1044650 and APP1003108; the Queensland Government’s Smart State Health and Medical Research Fund; the Princess Alexandra Hospital Research and Development Foundation and The Australian Liver Foundation; Irvine KM is the recipient of the Australian Liver Foundation Pauline Hall Fellowship; Powell EE is the recipient of an NHMRC Practitioner Fellowship, APP1004242; Sweet MJ is the recipient of an Australian Research Council (ARC) Future Fellowship, FT100100657; and an honorary NHMRC Senior Research Fellowship, APP1003470; Hill MM is the recipient of an ARC Future Fellowship, FT120100251
Correspondence to: Elizabeth E Powell, Professor, Director, Centre for Liver Disease Research, School of Medicine, The University of Queensland, Translational Research Institute, Brisbane, 37 Kent St, Woolloongabba, Brisbane 4102, Australia. e.powell@uq.edu.au
Telephone: +61-7-34438015 Fax: +61-7-34437779
Received: April 30, 2014 Revised: June 13, 2014 Accepted: July 16, 2014 Published online: December 21, 2014 Processing time: 234 Days and 9.4 Hours
Core Tip
Core tip: Hepatocyte senescence is observed in all chronic liver diseases of hepatocellular origin, even at early stages of disease progression. Although widely studied in cancer biology, the role of cellular senescence in the pathogenesis of inflammatory diseases is not known. We developed a novel model of stress-induced hepatocyte senescence and used it to demonstrate that senescent human hepatocytes adopt a hyper-secretory phenotype, which is likely to condition their microenvironment and contribute to disease pathogenesis. We used microarray and proteomic analysis to characterise senescent hepatocytes and identify candidate mediators; and confirmed the functional relevance of senescence-associated secretory phenotype by demonstrating that conditioned media from senescent hepatocytes elicits inflammatory macrophage migration.
