Caspase-1 inhibition alleviates acute renal injury in rats with severe acute pancreatitis

Table 1 Serum levels of blood urea nitrogen, creatinine, interleukin-1β and average gray value of interleukin-1β

Group	n	BUN (mmol/L)	Cr (mmol/L)	IL-1β(pg/mL)	IL-1βstaining (AGV)
HC	6	5.02 ± 0.42	41.17 ± 2.48	90.13 ± 21.13	63.67 ± 6.19
SAP-S
6 h	6	12.48 ± 2.30b	82.83 ± 13.89b	276.77 ± 44.92b	45.00 ± 4.98b
12 h	6	23.53 ± 2.58b	123.67 ± 17.67b	308.99 ± 34.95b	42.33 ± 4.89b
18 h	6	23.60 ± 3.33b	125.33 ± 21.09b	311.60 ± 46.51b	38.33 ± 5.13b
SAP-ICE-I
6 h	6	11.17 ± 1.71b	73.50 ± 11.62b	151.42 ± 27.26bd	53.00 ± 6.60bc
12 h	6	18.45 ± 2.66bd	75.67 ± 14.11bd	152.47 ± 29.60bd	49.67 ± 5.39bc
18 h	6	19.23 ± 2.18bd	77.50 ± 13.90bd	175.45 ± 29.72bd	46.50 ± 5.24bc

Values are mean ± SD;

^bP < 0.01 vs HC;

^cP < 0.05 and

^dP < 0.01 vs SAP-S. AGV: Average grey value; BUN: Blood urea nitrogen; Cr: Creatinine; HC: Healthy controls; SAP: Severe acute pancreatitis; SAP-ICE-I: SAP with IL-1β-converting-enzyme inhibition; SAP-S: SAP with saline.

Table 2 Intrarenal expression of caspase-1, interleukin-1β and interleukin-18 mRNAs

Group	n	Caspase-1	IL-1β	IL-18
HC	6	0.36 ± 0.03	0.13 ± 0.01	0.38 ± 0.03
SAP-S
6 h	6	0.45 ± 0.03b	0.19 ± 0.02b	0.57 ± 0.04b
12 h	6	0.46 ± 0.04b	0.20 ± 0.03b	0.58 ± 0.05b
18 h	5	0.43 ± 0.04b	0.20 ± 0.03b	0.55 ± 0.04b
SAP-ICE-I
6 h	6	0.45 ± 0.03b	0.15 ± 0.02d	0.45 ± 0.04bd
12 h	6	0.44 ± 0.03b	0.15 ± 0.02d	0.45 ± 0.04bd
18 h	5	0.45 ± 0.02b	0.15 ± 0.02d	0.44 ± 0.04bd

Values are mean ± SD;

^bP < 0.01 vs HC;

^dP < 0.01 vs SAP-S. HC: Healthy controls; SAP: Severe acute pancreatitis; SAP-ICE-I: SAP with IL-1β-converting-enzyme inhibition; SAP-S: SAP with saline.