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©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 28, 2014; 20(16): 4723-4729
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4723
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4723
Table 1 Primers for polymerase chain reaction
Primers | Sequences | Annealing temp (°C) | Size (bp) |
MT1M | |||
U | 5'-TTGAAAATGGTGGGGTGA-3' | 54 | 163 |
5'-AAACTATACACCAAATAATACACAATATCC-3' | |||
M | 5'-GACGTTCGCGACGTTAAG-3' | 54 | 124 |
5'-ACGCCGAATAATACGCAAT-3' | |||
MT1G | |||
U | 5'-GGGGTTGTTTTGTGGTGTGTG-3' | 59 | 135 |
5'-AAACACCCCACCCCACCCTT-3' | |||
M | 5'-TTCGCGAGTCGGTGCGAAAG-3' | 50 | 96 |
5'-CCGCGATCCCGACCTAAACT-3' |
Table 2 Clinicopathological data and serum metallothionein 1M and metallothionein 1G promoter methylation in hepatocellular carcinoma patients
Characteristics | n | MT1M | MT1G | ||||
1M | 2U | P value | 1M | 2U | P value | ||
Total number | 121 | 59 | 62 | 85 | 36 | ||
Gender | |||||||
Male | 100 | 50 | 50 | 0.552 | 70 | 30 | 0.896 |
Female | 21 | 9 | 12 | 15 | 6 | ||
Age (yr) | |||||||
≥ 55 | 67 | 32 | 35 | 0.807 | 46 | 21 | 0.670 |
< 55 | 54 | 27 | 27 | 39 | 15 | ||
HBV infection | |||||||
Yes | 100 | 50 | 50 | 0.552 | 69 | 31 | 0.512 |
No | 21 | 9 | 12 | 16 | 5 | ||
Vascular invasion or metastasis | |||||||
Yes | 44 | 26 | 18 | 0.086 | 33 | 11 | 0.387 |
No | 77 | 33 | 44 | 52 | 25 | ||
Histological differentiation | |||||||
Poor | 41 | 18 | 23 | 0.610 | 26 | 15 | 0.426 |
Moderate | 50 | 27 | 23 | 38 | 12 | ||
Well | 30 | 14 | 16 | 21 | 9 | ||
TNM stage | |||||||
I-II | 64 | 26 | 38 | 0.058 | 44 | 13 | 0.115 |
III-IV | 57 | 33 | 24 | 41 | 23 | ||
Tumor multiplicity | |||||||
Single | 72 | 26 | 36 | 0.741 | 52 | 20 | 0.565 |
Multiple | 49 | 23 | 26 | 33 | 16 | ||
Tumor size(cm) | |||||||
≥ 5 | 59 | 38 | 21 | 0.001 | 38 | 21 | 0.170 |
< 5 | 62 | 21 | 41 | 47 | 15 |
Table 3 Correlation of metallothionein 1M and metallothionein 1G promoter methylation with tumor size
Gene | Tumor size (cm) | rs | Pvalue | |
MethylatedM (P25-P75) | UnmethylatedM (P25-P75) | |||
MT1M | 6.5 (4.0-9.0) | 3.9 (2.2-6.4) | 0.321 | 0.000 |
MT1G | 4.4 (2.9-8.0) | 5.0 (3.5-7.9) | -0.049 | 0.590 |
Table 4 Vascular invasion or metastasis and a combination of metallothionein 1M and metallothionein 1G promoter methylation
Characteristic | n | MT1M and MT1G | ||
1M | 2U | P value | ||
Vascular invasion or metastasis | ||||
Yes | 44 | 18 | 26 | 0.018 |
No | 77 | 16 | 61 |
Table 5 Sensitivity and specificity of gene sets for hepatitis B virus (+) hepatocellular carcinoma detection in chronic hepatitis B group
No. | Marker | TP/FN | FP/TN | Sensitivity (%)TP/(TP + FN) | Specificity (%)TN/(TN + FP) |
1 | AFP | 56/44 | 14/23 | 56.0 | 62.1 |
2 | MT1M | 50/50 | 2/35 | 50.0 | 94.6 |
3 | MT1G | 69/31 | 6/31 | 69.0 | 83.8 |
4 | MT1M/MT1G | 90/10 | 7/30 | 90.0 | 81.1 |
Table 6 Sensitivity and specificity of gene sets for hepatocellular carcinoma detection in normal controls group
No. | Gene | TP/FN | FP/TN | Sensitivity (%)TP/(TP+FN) | Specificity (%)TN/(TN+FP) |
1 | MT1M | 59/62 | 2/29 | 48.8 | 93.5 |
2 | MT1G | 85/36 | 4/27 | 70.2 | 87.1 |
3 | MT1M/MT1G | 110/11 | 5/26 | 90.9 | 83.9 |
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Citation: Ji XF, Fan YC, Gao S, Yang Y, Zhang JJ, Wang K.
MT1M andMT1G promoter methylation as biomarkers for hepatocellular carcinoma. World J Gastroenterol 2014; 20(16): 4723-4729 - URL: https://www.wjgnet.com/1007-9327/full/v20/i16/4723.htm
- DOI: https://dx.doi.org/10.3748/wjg.v20.i16.4723