Brief Article
Copyright ©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 28, 2014; 20(16): 4712-4717
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4712
Table 1 Nucleotide sequences of the primers used for real-time reverse transcription polymerase chain reaction
GenesForward (5'-3'), reverse (5'-3')
Ptch1TGGTCACACGAACAATGG
TGAACTGGGCAGCTATGAAGTC
SmoAGTTACATCGCAGCCTTC
CACACTACTCCAGCCATC
Gli1TGCTGACACTCTGGGATA
CAGGGCCATAGTTGGTT
Table 2 Expression of Ptch, Smo, and Gli1 mRNA (mean ± SD)
RatsPtchSmoGli
Ethanol and glycerol mixture treated0.23 ± 0.160.14 ± 0.050.57 ± 0.12
DBTC treated2.38 ± 0.423.85 ± 1.034.63 ± 1.49