siRNA-targeted inhibition of growth hormone receptor in human colon cancer SW480 cells

doi:10.3748/wjg.v19.i44.8108

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Nov 28, 2013 (publication date) through Nov 3, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Nov 28, 2013; 19(44): 8108-8113
Published online Nov 28, 2013. doi: 10.3748/wjg.v19.i44.8108

Table 1 Weight changes of tumor-bearing mice (n = 6; mean ± SD)

	Weight (g, mean ± SD)
Time	NS	G₂	GH	FU	FU+G₂	G₂+FU+GH
Pre-operation	20.69 ± 0.67	21.92 ± 0.70	20.67 ± 0.57	21.93 ± 0.58	21.86 ± 0.73	21.25 ± 0.79
Inoculation 2 d	20.64 ± 0.60	21.86 ± 0.79	20.82 ± 0.56	20.89 ± 0.66¹	20.09 ± 0.55¹³	20.41 ± 0.85¹³
Inoculation 5 d	20.65 ± 0.49	21.56 ± 0.81	20.96 ± 0.54¹	20.94 ± 0.67¹	19.92 ± 0.58¹³⁵	20.42 ± 0.76¹
Inoculation 8 d	20.65 ± 0.65	21.53 ± 0.56¹	21.18 ± 0.44¹	20.41 ± 0.73¹³	19.92 ± 0.52¹²³⁵	20.53 ± 0.70¹³
Inoculation 11 d	20.67 ± 0.63	21.53 ± 0.73¹	21.27 ± 0.53¹⁴	19.86 ± 0.57¹³	20.06 ± 0.52¹²³⁵	20.63 ± 0.81¹⁵
Inoculation 14 d	20.61 ± 0.62	21.60 ± 0.68¹²	21.51 ± 0.44¹⁴	9.46 ± 0.52¹²³	19.86 ± 0.92¹²	20.86 ± 0.72¹⁴
Inoculation 17 d	20.68 ± 0.66	21.60 ± 0.71¹	21.64 ± 0.45¹²⁴	18.94 ± 0.47¹²³	19.40 ± 0.60¹²³	21.04 ± 0.78¹⁴

¹Compared with pre-operation, P < 0.05;

²Compared with NS group, P < 0.05;

³Compared with G₂ group, P < 0.05;

⁴Compared with FU group, P < 0.05;

⁵Compared with GH group, P < 0.05. NS: Normal saline; G₂: Recombinant plasmid; GH: Growth hormone; FU: 5-fluorouracil.

Table 2 Tumor volume changes in tumor-bearing mice (n = 6, mean ± SD)

	Subcutaneous tumor volume (mm³, mean ± SD)
Time	NS	G₂	GH	FU	FU+G₂	G₂+FU+GH
Inoculation 5 d	7.72 ± 1.61	7.93 ± 1.74	8.11 ± 1.65	7.42 ± 1.51	6.51 ± 1.20	7.33 ± 1.32
Inoculation 8 d	20.19 ± 4.91²³	13.44 ± 4.12¹³	33.28 ± 3.24¹²³	17.51 ± 5.75³	15.12 ± 5.01³	15.44 ± 4.23
Inoculation 11 d	106.02 ± 6.61²³	21.12 ± 4.04¹³	151.90 ± 8.31¹²³	21.00 ± 5.07¹³	19.22 ± 4.33¹³	22.97 ± 4.95¹²³
Inoculation 14 d	133.41 ± 6.43²³	20.00 ± 4.75¹³	178.93 ± 3.11¹²³	16.23 ± 6.51¹³	11.55 ± 4.11¹²³	12.12 ± 3.11¹²³
Inoculation 17 d	116.81 ± 0.61²³	9.71 ± 3.82¹³	149.01 ± 3.02¹²³	11.54 ± 2.42¹³	11.42 ± 1.11¹³	10.47 ± 1.02¹³

¹Compared with NS group, P < 0.05;

²Compared with G₂ group, P < 0.05;

³Compared with GH group, P < 0.05. NS: Normal saline; G₂: Recombinant plasmid; GH: Growth hormone; FU: 5-fluorouracil.

Table 3 △CT value of growth hormone receptor mRNA expression detected by real-time reverse transcription-polymerase chain reaction in all groups

	NS	LP	Negative	GH	G₂	FU	FU+G₂	G₂+FU+GH
△CT	12.63 ± 0.14	12.63 ± 0.43	12.67 ± 0.21	12.71 ± 0.39	14.12 ± 0.10¹	15.15 ± 0.44¹	16.46 ± 0.27¹²	15.37 ± 0.57¹²

¹Compared with NS group, P < 0.05;

²Compared with G₂ group, P < 0.05. NS: Normal saline; G₂: Recombinant plasmid; GH: Growth hormone; FU: 5-fluorouracil.

Citation: Zhou D, Yang J, Huang WD, Wang J, Zhang Q. siRNA-targeted inhibition of growth hormone receptor in human colon cancer SW480 cells. World J Gastroenterol 2013; 19(44): 8108-8113
URL: https://www.wjgnet.com/1007-9327/full/v19/i44/8108.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i44.8108