Abnormal DNA-PKcs and Ku 70/80 expression may promote malignant pathological processes in gastric carcinoma

Table 1 Differential immunohistochemical positive rate of DNA-dependent protein kinase and Ku 70/80 in gastric carcinoma and normal gastric mucosa tissues

Group	n	DNA-PKcs				P value	Ku 70/80				P value
		-	+	++	+++		-	+	++	+++
NGM	34	34	0	0	0	< 0.01	7	12	12	3	< 0.01
GC	146	9	24	65	48		5	19	50	72
H. pylori positive
NGM	16	16	0	0	0	< 0.01	2	4	8	2	< 0.10
GC	57	3	6	26	22		1	4	19	33
H. pylori negative
NGM	18	18	0	0	0	< 0.01	5	8	4	1	< 0.01
GC	89	6	18	39	26		4	15	31	39

H. pylori: Helicobacter pylori; GC: Gastric carcinoma; NGM: Normal gastric mucosa; DNA-PKcs: DNA-dependent protein kinase.

Table 2 Correlation between clinicopathological parameters and the expression of DNA-dependent protein kinase and Ku 70/80 in gastric carcinoma by Spearman analysis

Clinicopathological parameters	DNA-PKcs		Ku 70/80
	r	P value	r	P value
Age (yr)	0.557	0.049	0.083	0.317
Sex	0.073	0.382	-0.052	0.531
H. pylori infection	-0.139	0.102	-0.168	0.043
Lesion location	0.131	0.115	0.107	0.200
Histological type	0.046	0.583	0.008	0.922
Local Invasive	0.042	0.615	-0.052	0.534
Differentiation degree	-0.447	< 0.001	0.068	0.414
Lymph node metastasis	0.029	0.729	0.123	0.139
Clinical stages	0.010	0.902	-0.189	0.022

H. pylori: Helicobacter pylori; DNA-PKcs: DNA-dependent protein kinase.

Table 3 Expression of DNA-dependent protein kinase and Ku 70/80 in relation to the clinicopathological parameters of patients with gastric carcinoma

Clinicopathological parameters	n	DNA-PKcs				P value	Ku 70/80				P value
		-	+	++	+++		-	+	++	+++
Age (yr)						0.555					0.315
< 55	60	5	9	28	18		2	10	21	27
≥ 55	86	4	15	37	30		3	9	29	45
Sex						0.380					0.530
Male	96	5	20	40	31		3	10	35	48
Female	50	4	4	25	17		2	9	15	24
H. pylori infection						0.116					0.044
Positive	57	3	6	26	22		1	4	19	33
Negative	89	6	18	39	26		4	15	31	39
Lesion location						0.114					0.199
Antrum	74	3	16	36	19		3	8	32	31
Others	72	6	8	29	29		2	11	18	41
Histological type
Protrude1	15	3	1	6	5	0.8401-2	0	4	4	7	0.6231-2
Ulcerative2	57	2	10	29	16	0.5861-3					0.5461-3
Infiltrative ulcer3	59	4	9	24	22	0.7921-4					0.9111-4
						0.9462-3	2	6	21	28	0.8942-3
Diffuse infiltration4	15	0	4	6	5	0.9462-4	1	6	23	29	0.6552-4
						0.7913-4	2	3	2	8	0.5913-4
Local invasion
Mucosa and submucosa1	14	2	2	6	4	0.6421-2	0	1	5	8	0.5011-2
Muscular layer2	21	1	4	9	7	0.5181-3	1	2	8	10	0.3951-3
Serosa and subserosa3	111	6	18	50	37	0.9262-3	4	16	37	54	0.9542-3
Differentiation degree
G11	64	3	3	22	36	0.0001-2	1	6	24	33	0.3211-2
G22	57	4	13	29	11	0.0001-3	3	6	23	25	0.6181-3
G33	25	2	8	14	1	0.1462-3	1	7	3	14	0.8662-3
Lymph node metastasis						0.728					0.318
Positive	113	6			36		5	15	41	52
Negative	33	3			12		0	4	9	20
Clinical stages
I1	13	2			4	0.5391-2	0	1	5	7	0.6731-2
II2	46	2			14	0.5451-3	2	3	12	29	0.3821-3
III3	34	1			12	0.6381-4	0	3	18	13	0.2431-4
IV4	53	4			18	0.9832-3	3	12	15	23	0.0752-3
						0.8322-4					0.0292-4
						0.8203-4					0.5113-4

^1-4Distinguish the P value between each histological type. DNA-PKcs: DNA-dependent protein kinase.