Expression and significance of Musashi-1 in gastric cancer and precancerous lesions

Table 1 Expression intensity of Musashi-1 and proliferating cell nuclear antigen in different pathological tissues

Pathological tissues	Msi-1 protein staining intensity (n)				PCNA protein staining intensity (n)
	-	+	++	+++	-	+	++	+++
Normal gastric mucosa	17	19	0	0	27	9	0	0
Intestinal metaplasiaab	7	21	3	0	0	9	10	12
Low grade intraepithelial neoplasia	21	32	4	0	0	9	29	19
High grade intraepithelial neoplasia	19	17	4	1	0	6	16	19
Intestinal-type GCcd	12	29	9	4	0	0	10	44

^aP < 0.05,

^bP < 0.01, significantly different vs normal gastric mucosa;

^cP < 0.05,

^dP < 0.01, significantly different vs high-grade intraepithelial neoplasia. PCNA: Proliferating cell nuclear antigen; Msi-1: Musashi-1; GC: Gastric cancer.

Table 2 Relationship between Musashi-1 expression and clinicopathological parameters

Variable	Group	No.	Msi-1 staining intensity
			–	+	++	+++
Infiltration depth	≤ T1	20	8	9	3	0
	T2-4	34	4	20	6	4
Lymph node metastasisa	NO	27	11	9	5	2
	YES	27	1	20	4	2
Distant metastasis	NO	40	12	21	5	2
	YES	14	0	8	4	2
TNM stagingb	Stage I	27	11	10	4	2
	Stage II-IV	27	1	19	5	2

There was a significant difference between Musashi-1 (Msi-1) expression and clinicopathological parameters in Gastric cancer.

^aP < 0.01 and

^bP < 0.05. TNM: Tumor node metastasis.

Table 3 Relationship between Musashi-1 and proliferating cell nuclear antigen expression

Msi-1 protein staining intensity	PCNA protein staining intensity				Total
	Negative	Weak positive	Positive	Strong positive
Negative	10	23	17	26	76
Weak positive	17	7	40	54	118
Positive	0	2	7	11	20
Strong positive	0	1	1	3	5
Total	27	33	65	94	219

PCNA: Proliferating cell nuclear antigen; Msi-1: Musashi-1.