Gastric antisecretory and antiulcer activity of bovine hemoglobin

Table 1 Effect of bovine hemoglobin on the volume of gastric secretion and acidity in 6 h pylorus ligated rats

Treatment	Dose of B-Hb	Gastric secretion	Total acid output
	(mg/kg)	(mL)	(mEq)
Control	0	8.1 ± 0.61	498.7 ± 31.8
Hemoglobin	100	5.6 ± 0.63a	471.1 ± 75.0
Hemoglobin	300	5.5 ± 0.75a	438.4 ± 65.6
Hemoglobin	900	4.7 ± 0.58b	287.6 ± 24.6a

Values are mean ± SE.

^aP < 0.05,

^bP < 0.01 vs control group using Dunnett’s multiple test. B-Hb: Bovine hemoglobin.

Table 2 Effect of bovine hemoglobin on ethanol induced changes in Alcian blue binding capacity, non-protein sulfhydryl groups levels and myeloperoxidase in gastric mucosa of rats

Treatment	Dose of B-Hb (mg/kg)	Alcian blue binding (mg/g tissue)	Non-protein sulfhydryl (mmol/g tissue)	Myeloperoxidase activity (DA/g tissue)
Control	0	667 ± 25.8	4.59 ± 0.29	15.94 ± 1.6
EtOH alone	1 mL/animal	480 ± 25.6b	1.60 ± 0.12b	24.54 ± 1.9b
EtOH ± B-Hb	100	516 ± 31.6	3.11 ± 0.35d	24.30 ± 4.2
EtOH ± B-Hb	300	558 ± 28.8d	3.73 ± 0.42d	14.25 ± 2.6c
EtOH ± B-Hb	900	654 ± 33.8d	5.36 ± 0.34d	17.21 ± 0.98c

Values are mean ± SE.

^bP < 0.01 vs control;

^cP < 0.05,

^dP < 0.01 vs EtOH group using Dunnett’s multiple comparison test. B-Hb: Bovine hemoglobin.