Copyright
©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Jun 7, 2013; 19(21): 3226-3240
Published online Jun 7, 2013. doi: 10.3748/wjg.v19.i21.3226
Published online Jun 7, 2013. doi: 10.3748/wjg.v19.i21.3226
Table 1 Oligonucleotide sequences used in real-time polymerase chain reaction
Gene | Primer (5' to 3') | Length (bp) |
β-actin | F: 5'TGGAGAAAATCTGGCACCA3' | 189 |
R: 5'CAGGCGTACAGGGATAGCAC3' | ||
p21 | F: 5'TCCAAGAGGAAGCCCTAATCC3' | 101 |
R: 5'ACAAACTGAGACTAAGGCAGAAGATG3' | ||
p53 | F: 5'TCAGTCTACCTCCCGCCATAA3' | 231 |
R: 5'GTGCAGGCCAACTTGTTCAGT3' | ||
Bcl-2 | F: 5'CCTTTTCTACTTTGCCAGCAAAC3' | 149 |
R: 5'GAGGCCGTCCCAACCAC3' | ||
CDK2 | F: 5'GCTAGCAGACTTTGGACTAGCCAG3' | 85 |
R: 5'AGCTCGGTACCACAGGGTCA3' | ||
CyclinD1 | F: 5'AACAGAAGTGCGAGGAGGAG3' | 299 |
R: 5'CTGGCATTTTGGAGAGGAAG3' | ||
Bax | F: 5'CCAGGGTGGTTGGGTGAGACT3' | 231 |
R: 5'TGGGAGGTCAGCAGGGTAGAT3' |
Table 2 Proteomic identification of acetylated proteins in AGS cells
Reference | Sequence |
Filamin A, alpha | K.TGVAVNKPAEFTVDAK*HGGK.A |
Heat shock cognate 71 kDa protein | R.RFDDAVVQSDMK*HWPFMVVNDAGRPK.H |
Heat shock protein HSP 90-alpha | R.MKENQK*HIYYITGETK.D |
R.MK*ENQKHIYYITGETK.D | |
Histone H2B type 1-C/E/F/G/I | M.PEPAK*SAPAPK*K*GSK*K*AVTK*AQK.K |
R.LLLPGELAK*HAVSEGTK.A | |
Histone H2B type 1-D | K.SAPAPK*K*GSK*K*AVTK*AQK*K.D |
Histone H2B type 1-H | K.#KGSK*K*AVTK*AQK*K.D |
Histone H2B type 2 | K.SAPAPK*K*GSK*K*AVTK*VQK.K |
Ezrin | R.QAVDQIK*SQEQLAAELAEYTAK.I |
Histone H4 | M.SGRGK*GGK*GLGK*GGAK*R.H |
Fructose-bisphosphate aldolase A | K.DGADFAK*WR.C |
Histone H2B type 1-B | K.K*GSK*K*AITK*AQK*K.D |
K.SAPAPK*K*GSK*K*AITK*AQK.K | |
R.LLLPGELAK*HAVSEGTK.A | |
Tubulin beta chain | R.ISVYYNEATGGK*YVPR.A |
Histone H2B type 1-M | K.K*GSK*K*AINK*AQK.K |
T-complex protein 1 subunit theta | K.EGAK*HFSGLEEAVYR.N |
Tubulin beta-4B chain | R.INVYYNEATGGK*YVPR.A |
Uncharacterized protein | K.HELQANCYEEVK*DR.C |
Histone H3 | R.K*QLATK*AAR.K |
R.K*STGGK*APR.K | |
Glyceraldehyde-3-phosphate dehydrogenase | R.VIISAPSADAPMFVMGVNHEK*YDNSLK.I |
Heterogeneous nuclear ribonucleoprotein Q | K.SAFLCGVMK*TYR.Q |
Phosphoglycerate kinase 1 | R.FHVEEEGK*GK.D |
Histone H3.1 | R.EIAQDFK*TDLR.F |
T-complex protein 1 subunit alpha | K.DDK*HGSYEDAVHSGALND.- |
Phosphoglycerate mutase 1 | K.AETAAK*HGEAQVK.I |
Nucleophosmin | K.VEAK*FINYVK.N |
60S ribosomal protein L3 | K.FIDTTSK*FGHGR.F |
ADP/ATP translocase 3 | K.QIFLGGVDK*HTQFWR.Y |
U1 small nuclear ribonucleoprotein 70 kDa | R.VNYDTTESK*LR.R |
ATP5O ATP synthase subunit O, mitochondrial | GEVPCTVTSASPLEEATLSELK*TVLK |
CREB-binding protein | K.#KK*NNK*K*TNK*NK*SSISR.A |
K.SHAHK*MVK*WGLGLDDEGSSQGEPQSK*SPQESR.R | |
R.KKEESTAASETTEGSQGDSK*NAK*K.K | |
HUMAN Histone H2A.Z | M.AGGK*AGK*DSGK*AK*TK.A |
Uncharacterized protein | K.FK*YDDAER.R |
Uncharacterized protein | K.SAFLCGVMK*TYR.Q |
Heat shock protein beta-1 | K.DGVVEITGK*HEER.Q |
T-complex protein 1 subunit beta | R.EALLSSAVDHGSDEVK*FR.Q |
Galectin-1 | K.LPDGYEFK*FPNR.L |
Histone H2A type 1-H | R.GK*QGGK*AR.A |
Asparagine synthetase | K.VASVEMVK*YHHCR.D |
Retinoblastoma binding protein 7 | K.IECEIK*INHEGEVNR.A |
Glucose-6-phosphate isomerase | R.SGDWK*GYTGK.T |
Uncharacterized protein | R.EQCCYNCGK*PGHLAR.D |
Uncharacterized protein | K.IASK*YDHQAEEDLR.N |
Actin-related protein 3 | K.EFNK*YDTDGSK.W |
Histone H2A type 2-B | R.GK*QGGK*AR.A |
Uncharacterized protein | K.ITIMPK*.H |
Programmed cell death protein 5 | K.HGDPGDAAQQEAK*HR.E |
Fumarate hydratase, mitochondrial | K.VPNDK*YYGAQTVR.S |
Lamin-B receptor | K.YGVAWEK*YCQR.V |
Uncharacterized protein | K.VTGTLETK*YK.W |
PC4 and SFRS1-interacting protein | K.TKDQGK*K*GPNK*K*.L |
26S protease regulatory subunit 10B | K.VVSSSIVDK*YIGESAR.L |
Hematological and neurological expressed 1 protein | R.RNPPGGK*SSLVLG.- |
Uncharacterized protein | R.LNQVIFPVSYNDK*FYK.D |
Acyl-CoA-binding protein | K.TK*PSDEEM@LFIYGHYK.Q |
Probable global transcription activator SNF2L2 | K.K*GK*GGAK.T |
Chromodomain-helicase-DNA-binding protein 4 | R.NLGK*GK.R |
Biorientation of chromosomes in cell division protein 1-like | K.SLLEEK*LVLK*SK*.S |
Aspartate aminotransferase | R.DVFLPK*PTWGNHTPIFR.D |
Histone-lysine N-methyltransferase MLL3 | K.TLVLSDK*HSPQK*K.S |
Bromodomain-containing protein 1 | R.HPSSPCSVK*HSPTR.E |
LINE-1 type transposase domain-containing protein 1 | R.KFQK*LKNKEEVLK*.A |
B-cell CLL/lymphoma 9-like protein | R.GHCPPAPAK*PMHPENK*LTNHGK.T |
WD repeat-containing protein 46 | M.ETAPK*PGK.D |
Metastasis-associated protein MTA2 | R.VGCK*YQAEIPDR.L |
Cyclin-dependent kinase 11B | R.SHSAEGGK*HAR.V |
Homeobox protein Hox-C8 | R.YQTLELEK*.E |
Ubiquitin carboxyl-terminal hydrolase 8 | R.KEEQEQK*AKK*K.Q |
28S ribosomal protein S9, mitochondrial | K.AEAIVYK*HGSGR.I |
GDNF family receptor alpha-like | K.K*CINKSDNVK*EDK*FK.W |
Solute carrier organic anion transporter family member 4C1 | K.FGK*SIK.D |
Ovochymase-1 | R.#GAFGISYIDLK*VLGPK.D |
Coiled-coil domain-containing protein 13 | K.KKIEEDRFAFTGTAGVAGDVVATK*.I |
Threonine synthase-like 1 | K.LSCGEWK*SLVGATYVER.A |
Secreted frizzled-related protein 1 | K.K*K*DLKKLVLYLK*.N |
Dynein heavy chain 3, axonemal | R.LREAEGKLAAQMQK*.L |
Histone acetyltransferase KAT6B | R.#QSPAKVQSK*NK*YLHSPESR.P ! |
Polycomb protein SCMH1 | R.KPGK*K*R.G |
Zinc finger protein 512B | K.#EK*K*K*NLAGGK*K*.R |
Dedicator of cytokinesis protein 7 | R.AHGELHEQFK*R.K |
Remodeling and spacing factor 1 | R.GK*DISTITGHR.G |
HIRA-interacting protein 3 | R.#TQLKGGK*R.L |
Ubiquitin-fold modifier-conjugating enzyme 1 | K.ICLTDHFK*PLWAR.N |
Uncharacterized protein | K.QNKTK*R.Q |
Chromosome 1 open reading frame 149 | R.YLTNQK*NSNSK*NDR.R |
Uncharacterized protein | K.#EK*GDK*K*EGKDVK*.V |
Uncharacterized protein | K.NLACEESK*R.K |
Ubiquitin carboxyl-terminal hydrolase | R.DLLQFFK*PR.Q |
Uncharacterized protein | R.YLVASK*.E |
- Citation: Wang YG, Wang N, Li GM, Fang WL, Wei J, Ma JL, Wang T, Shi M. Mechanisms of trichostatin A inhibiting AGS proliferation and identification of lysine-acetylated proteins. World J Gastroenterol 2013; 19(21): 3226-3240
- URL: https://www.wjgnet.com/1007-9327/full/v19/i21/3226.htm
- DOI: https://dx.doi.org/10.3748/wjg.v19.i21.3226