Wang YG, Wang N, Li GM, Fang WL, Wei J, Ma JL, Wang T, Shi M. Mechanisms of trichostatin A inhibiting AGS proliferation and identification of lysine-acetylated proteins. World J Gastroenterol 2013; 19(21): 3226-3240 [PMID: 23745024 DOI: 10.3748/wjg.v19.i21.3226]
Corresponding Author of This Article
Min Shi, MD, PhD, Department of Gastroenterology, Shanghai Changning Central Hospital, No. 1111 Xianxia Road, Changning district, Shanghai 200336, China. shimingdyx@yeah.net
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jun 7, 2013; 19(21): 3226-3240 Published online Jun 7, 2013. doi: 10.3748/wjg.v19.i21.3226
Mechanisms of trichostatin A inhibiting AGS proliferation and identification of lysine-acetylated proteins
Yu-Gang Wang, Na Wang, Guang-Ming Li, Wen-Li Fang, Jue Wei, Jia-Li Ma, Ting Wang, Min Shi
Yu-Gang Wang, Na Wang, Wen-Li Fang, Jue Wei, Jia-Li Ma, Ting Wang, Min Shi, Department of Gastroenterology, Shanghai Changning Central Hospital, Shanghai 200336, China
Guang-Ming Li, Department of Gastroenterology, Xinhua Hospital, Shanghai Second Medical University, Shanghai 200092, China
Author contributions: Wang YG, Wang N and Shi M designed the study; Wang YG, Wang N, Li GM, Fang WL, Wei J, Wang T and Shi M carried out the study; Wang YG, Wang N, Li GM, Fang WL, Wei J, Wang T and Shi M contributed new reagents and analytic tools; Wang YG, Wei J and Ma JL analyzed the data; Wang YG, Wang N and Shi M wrote the paper.
Supported by Shanghai Municipal Health Bureau Key Disciplines Grant, No. ZK2012A05; National Natural Science Foundation, No. 81070344
Correspondence to: Min Shi, MD, PhD, Department of Gastroenterology, Shanghai Changning Central Hospital, No. 1111 Xianxia Road, Changning district, Shanghai 200336, China. shimingdyx@yeah.net
Telephone: +86-21-62909911 Fax:+86-21-62906478
Received: January 16, 2013 Revised: March 21, 2013 Accepted: April 9, 2013 Published online: June 7, 2013 Processing time: 149 Days and 12.6 Hours
Core Tip
Core tip: Previous research has shown that deacetyltransferase inhibitors not only induce cell cycle arrest, differentiation and apoptosis of many tumor cells in vitro, but also inhibit tumor growth in tumor-bearing animals. They are through the acetylation modification of deacetyltransferase inhibitor on histone. Only a few studies have focused on the acetylation modification by deacetyltransferase on non-histone. This is the first study to identify acetylated proteins in gastric cancer cells before and after exposure to trichostatin A to explore the effect of lysine acetylation of related proteins on the regulation of gastric cancer cell proliferation. Moreover, the lysine-acetylated proteins and the modified sites in AGS cells were assessed. We explored whether ATP5O was obviously acetylated after trichostatin A treatment in AGS cells.
