Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array

Table 1 Colorectal cancer cell lines origin

Cell line	Type/origin
SW620	Colon adenocarcinoma. Derived from: metastasis to lymph node
T84	Colon carcinoma. Derived from metastasis to lung
LoVo	Derived from metastatic site: left supraclavicular region
SW480	Colon adenocarcinoma
WiDr	Colon adenocarcinoma
RKO	Colon carcinoma
HT29	Colon adenocarcinoma
HCT15	Colon adenocarcinoma
HCT116	Colon carcinoma
SW1116	Colon adenocarcinoma
SW1417	Colon adenocarcinoma
LS174T	Colon adenocarcinoma
LS513	Colon carcinoma
Caco2	Colon adenocarcinoma
DLD-1	Colon adenocarcinoma
LS411N	Colon adenocarcinoma
Colo320	Colon adenocarcinoma

Table 2 Primer and probe sequences for vascular endothelial growth factor isoforms quantitative reverse-transcription polymerase chain reaction

	Sense primer	Antisense primer	Taqman probe	Amplicon size (bp)
VEGF end-point and cloning	ACTGCCATCCAATCGAGACC	GATGGCTTGAAGATGTACTCGATCT
GAPDH end-point and cloning	TGGTATCGTGGAAGGACTCATGAC	ATGCCAGTGAGCTTCCCGTTCAGC		189
VEGF121 mRNA	CAAGGCCAGCACATAGGAGA	CTCGGCTTGTCACATTTTTC	CTTCCTACAGCACAACAAATGTGAATGCAGA	101
VEGF165 mRNA	TGTGAATGCAGACCAAAGAAAGA	TGCTTTCTCCGCTCTGAGC	AGAGCAAGACAAGAAAATCCCTGTGGGC	74
VEGF189 mRNA	CGCAAGAAATCCCGGTATAAGT	TGCTTTCTCCGCTCTGAGC	AGGCCCACAGGGAACGCTCCAG	65
GAPDH	TGGTATCGTGGAAGGACTCATGAC	ATGCCAGTGAGCTTCCCGTTCAGC	CCCAGAGACTGTGGATGGCCCC	189

VEGF: Vascular endothelial growth factor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 3 Association between vascular endothelial growth factor mRNA isoforms and vascular endothelial growth factor protein secretion

	VEGF protein	VEGF121 mRNA	VEGF165 mRNA
VEGF121 mRNA	r = 0.55
	P = 0.034
VEGF165 mRNA	r = 0.67	r = 0.93
	P = 0.007	P = 0.000
VEGF189 mRNA	r = 0.69	r = 0.95	r = 0.92
	P = 0.005	P = 0.000	P = 0.000

VEGF: Vascular endothelial growth factor.