c-Jun N-terminal kinase is required for thermotherapy-induced apoptosis in human gastric cancer cells

Table 1 Primers for reverse transcription polymerase chain reaction

Gene	Primer sequence	Size (bp)	Annealing temperature
β-actin	F: TCAGGTCATCACTATCGGCAAT	432	57 °C
	R: AAAGAAAGGGTGTAAAAGGCA
JNK	F: CACAGTCCTAAAACGATACC	354	57 °C
	R: CCACACAGCATTTGATAGAG

JNK: c-Jun N-terminal kinase; F: Forward primer; R: Reverse primer.

Table 2 Cell cycle of SGC-7901 cells following thermotherapy for various time periods (%)

Group	SGC-7901 cells
	G0-G1	S	G2-M
0 h	27.22	62.47	10.31
1 h	71.15a	22.52a	6.33
2 h	70.89a	17.11a	12

^aP < 0.05 vs without thermotherapy treatment.

Table 3 Apoptotic rate of SGC-7901 cells with or without SP600125 treatment following thermotherapy for various time periods as determined by flow cytometry and terminal deoxynucleotidyl transferase dUTP nick end labeling assay

	Apoptotic rate(%)
Group (h)	Control group	SP600125 group
Flow cytometry
0	7.3 ± 0.10	3.2 ± 0.08
0.5	46.5 ± 0.23b	21.8 ± 0.15bd
1	39.9 ± 0.53b	17.9 ± 0.26bd
2	56.6 ± 0.35b	22.4 ± 0.36bd
3	50.4 ± 0.29b	24.5 ± 0.72bd
TUNEL assay
0	12.2 ± 0.22	11.3 ± 0.13
0.5	48.2 ± 0.40b	25.8 ± 0.19ad
1	40.1 ± 0.20b	19.2 ± 0.09ad
2	61.2 ± 0.29b	26.3 ± 0.23ad
3	52.0 ± 0.42b	23.4 ± 0.36ad

^aP < 0.05,

^bP < 0.01 vs without thermotherapy;

^dP < 0.01 vs without SP600125 treatment. TUNEL: Terminal deoxynucleotidyl transferase dUTP nick end labeling.