Single nucleotide polymorphisms in the CDH17 gene of colorectal carcinoma

doi:10.3748/wjg.v18.i48.7251

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 28, 2012; 18(48): 7251-7261
Published online Dec 28, 2012. doi: 10.3748/wjg.v18.i48.7251

Table 1 Primer sequences and fragment sizes of polymerase chain reaction products

Gene	SNPs site (refSNP )	Primer sequence(5’-3’)	Fragment sizes of PCR products (bp)
CDH17	c.343A>G (rs2243518)	Forward primer: CCAACATGGTTTCCTTTTCCTC	159
	c.343A>G (rs2243518)	Reverse primer: GTTCTGCCTTACTGAGCCTTCG	159
	c.2216A>C (rs1051624)	Forward primer: AATCCAGGGTCTGAAGTTGTA	198
	c.2216A>C (rs1051624)	Reverse primer: TACTAGCCTGAGTTGCCTATA	198

SNPs: Single nucleotide polymorphisms; PCR: Polymerase chain reaction.

Table 2 The correlation between the genotype of CDH17 gene c.343A>G and clinicopathologic parameters in colorectal carcinomas n (%)

Clinicopathologic parameters	n	*Genotype CDH17* c.343A>G**			χ²	P value
Clinicopathologic parameters	n	A/A	A/G	G/G	χ²	P value
Gender
Male	47	10 (21.3)	25 (53.2)	12 (25.5)	2.236	0.327
Female	46	16 (34.8)	19 (41.3)	11 (23.9)	2.236	0.327
Age (yr)
< 50	29	10 (34.5)	15 (51.7)	4 (13.8)	2.854	0.240
≥ 50	64	16 (25)	29 (45.3)	19 (29.7)	2.854	0.240
Tumor location
Colon	32	8 (25.0)	16 (50.0)	8 (25.0)	0.229	0.892
Rectum	61	18 (29.5)	28 (45.9)	15 (24.6)	0.229	0.892
Tumor size (cm)
< 5	68	18 (26.5)	34 (50.0)	16 (23.5)	0.734	0.693
≥ 5	25	8 (32.0)	10 (40.0)	7 (28.0)	0.734	0.693
Macroscopic appearance
Protrude type	31	8 (25.8)	17 (54.8)	6 (19.4)	1.948	0.745¹
Ulcer type	46	12 (26.1)	21 (45.7)	13 (28.3)
Infiltrating type	16	6 (37.5)	6 (37.5)	4 (25.0)
Colloid type	0	0 (0.0)	0 (0.0)	0 (0.0)
Histologic type
Adenocarcinoma	71	18 (25.4)	34 (47.9)	19 (26.8)	2.179	0.703¹
Adenocarcinoma and mucinous carcinoma	9	4 (44.4)	3 (33.3)	2 (22.2)
Mucinous carcinoma	13	4 (30.8)	7 (53.8)	2 (15.4)
Undifferentiated carcinoma	0	0 (0.0)	0 (0.0)	0 (0.0)
Adenosquamous carcinoma	0	0 (0.0)	0 (0.0)	0 (0.0)
Squamous cell carcinoma	0	0 (0.0)	0 (0.0)	0 (0.0)
Invasion depth
Submucosa	2	0 (0.0)	2 (100.0)	0 (0.0)	0.696	0.706²
Muscular layer	34	12 (35.3)	15 (44.1)	7 (20.6)
Serous coat	48	12 (25)	21 (43.8)	15 (31.3)
Other organs	9	2 (22.2)	6 (66.7)	1 (11.1)
Differentiation degree
Well-differentiated	4	0 (0.0)	2 (50.0)	2 (50.0)	2.212	0.331²
Moderately-differentiated	63	17 (27.0)	33 (52.4)	13 (20.6)
Poorly-differentiated	26	9 (34.6)	9 (34.6)	8 (30.8)
Lymph node metastasis
No	59	16 (27.1)	35 (59.3)	8 (13.6)	13.105	0.001³
Yes	34	10 (29.4)	9 (26.5)	15 (44.1)	13.105	0.001³
TNM grade
I	26	8 (30.8)	16 (61.5)	2 (7.7)	10.344	0.006²³
II	31	7 (22.6)	18 (58.1)	6 (19.4)
III	33	9 (27.3)	9 (27.3)	15 (45.5)
IV	3	2 (66.7)	1 (33.3)	0 (0.0)
Hematogenous metastasis
No	90	24 (26.7)	43 (47.8)	23 (25.6)	2.556	0.279¹
Yes	3	2 (66.7)	1 (33.3)	0 (0.0)	2.556	0.279¹
Recurrence
No	91	24 (26.4)	44 (48.4)	23 (25.3)	5.267	0.072¹
Yes	2	2 (100.0)	0 (0.0)	0 (0.0)	5.267	0.072¹

¹The Fisher’s Exact Test;

²The Kruskal-Wallis Test;

³Statistically significant.

Table 3 The correlation between the genotype of CDH17 gene c.2216A>C and clinicopathologic parameters in colorectal carcinomas n (%)

Clinicopathologic parameters	n	*Genotype CDH17* c.2216A>G**			χ²	P value
Clinicopathologic parameters	n	A/A	A/C	C/C	χ²	P value
Gender
Male	47	20 (42.6)	5 (10.6)	22 (46.8)	3.656	0.161
Female	46	11 (23.9)	7 (15.2)	28 (60.9)	3.656	0.161
Age (yr)
< 50	29	6 (20.7)	5 (17.2)	18 (62.1)	3.176	0.204
≥ 50	64	25 (39.1)	7 (10.9)	32 (50.0)	3.176	0.204
Tumor location
Colon	32	10 (31.3)	6 (18.8)	16 (50.0)	1.485	0.476
Rectum	61	21 (34.4)	6 (9.8)	34 (55.7)	1.485	0.476
Tumor size (cm)
< 5	68	25 (36.8)	5 (7.4)	38 (55.9)	7.144	0.028³
≥ 5	25	6 (24.0)	7 (28.0)	12 (48.0)	7.144	0.028³
Macroscopic appearance
Protrude type	31	11 (35.5)	4 (12.9)	16 (51.6)	1.025	0.906¹
Ulcer type	46	16 (34.8)	5 (10.9)	25 (54.3)
Infiltrating type	16	4 (25.0)	3 (18.8)	9 (56.3)
Colloid type	0	0 (0.0)	0 (0.0)	0 (0.0)
Histologic type
Adenocarcinoma	71	23 (32.4)	10 (14.1)	38 (53.5)	1.684	0.794¹
Adenocarcinoma and mucinous carcinoma	9	4 (44.4)	0 (0.0)	5 (55.6)
Mucinous carcinoma	13	4 (30.8)	2 (15.4)	7 (53.8)
Undifferentiated carcinoma	0	0 (0.0)	0 (0.0)	0 (0.0)
Adenosquamous carcinoma	0	0 (0.0)	0 (0.0)	0 (0.0)
Squamous cell carcinoma	0	0 (0.0)	0 (0.0)	0 (0.0)
Invasion depth
Submucosa	2	2 (100.0)	0 (0.0)	0 (0.0)	1.610	0.447²
Muscular layer	34	12 (35.3)	4 (11.8)	18 (52.9)
Serous coat	48	14 (29.2)	5 (10.4)	29 (60.4)
Other organs	9	3 (33.3)	3 (33.3)	3 (33.3)
Differentiation degree
Well-differentiated	4	0 (0.0)	0 (0.0)	4 (100.0)	0.090	0.956²
Moderately-differentiated	63	24 (38.1)	9 (14.3)	30 (47.6)
Poorly-differentiated	26	7 (26.9)	3 (11.5)	16 (61.5)
Lymph node metastasis
No	59	25 (42.4)	10 (16.9)	24 (40.7)	11.143	0.004³
Yes	34	6 (17.6)	2 (5.9)	26 (76.5)	11.143	0.004³
TNM grade
I	26	12 (46.2)	4 (15.4)	10 (38.5)	6.710	0.035²³
II	31	12 (38.7)	5 (16.1)	14 (45.2)
III	33	6 (18.2)	2 (6.1)	25 (75.8)
IV	3	1 (33.3)	1 (33.3)	1 (33.3)
Hematogenous metastasis
No	90	30 (33.3)	11 (12.2)	49 (54.4)	1.243	0.537¹
Yes	3	1 (33.3)	1 (33.3)	1 (33.3)	1.243	0.537¹
Recurrence
No	91	29 (31.9)	12 (13.2)	50 (54.9)	4.088	0.130¹
Yes	2	2 (100.0)	0 (0.0)	0 (0.0)	4.088	0.130¹

¹The Fisher’s Exact Test;

²The Kruskal-Wallis Test;

³Statistically significant.

Table 4 The correlation between the genotype and allele frequencies of CDH17 gene c.343A>G and lymph node metastasis n (%)

	Lymph node metastasis		OR (95%CI)
	No	Yes
Genotype			OR (95%CI)
A/G	35 (59.3)	9 (26.5)	1.000 (reference)
A/A	16 (27.1)	10 (29.4)	2.431 (0.828-7.139)
G/G	8 (13.6)	15 (44.1)	7.292 (2.360-22.532)
Allele frequency
A	67 (56.8)	29 (42.6)	1.000 (reference)
G	51 (43.2)	39 (57.4)	1.767 (0.967-3.229)

OR: Odds ratios.

Table 5 The correlation between the genotype and allele frequency of CDH17 gene c.343A>G and tumor-node-metastasis grade n (%)

	TNM grade				Mean rank
	I	II	III	IV	Mean rank
Genotype
A/G	16 (61.5)	18 (58.1)	9 (27.3)	1 (33.3)	39.32
A/A	8 (30.8)	7 (22.6)	9 (27.3)	2 (66.7)	48.15
G/G	2 (7.7)	6 (19.4)	15 (45.5)	0 (0.0)	60.39
Allele frequency
A	32 (61.5)	32 (51.6)	27 (40.9)	5 (83.3)	87.71
G	20 (38.5)	30 (48.4)	39 (59.1)	1 (16.7)	99.68

TNM: Tumor-node-metastasis.

Table 6 The correlation between the genotype and allele frequency of CDH17 gene c.2216A>C and lymph node metastasis n (%)

	Lymph node metastasis		OR (95%CI)
	No	Yes
Genotype			OR (95%CI)
A/C	10 (16.9)	2 (5.9)	1.000 (reference)
A/A	25 (42.4)	6 (17.6)	1.200 (0.206-6.977)
C/C	24 (40.7)	26 (76.5)	5.417 (1.076-27.272)
Allele frequency
A	60 (50.8)	14 (20.6)	1.000 (reference)
C	58 (49.2)	54 (79.4)	3.990 (2.002-7.953)

OD: Odds ratio.

Table 7 The correlation between the genotype and allele frequency of CDH17 gene c.2216A>C and tumor-node-metastasis grade n (%)

	TNM grade				Mean rank
	I	II	III	IV	Mean rank
Genotype
A/C	4 (15.4)	5 (16.1)	2 (6.1)	1 (33.3)	42
A/A	12 (46.2)	12 (38.7)	6 (18.2)	1 (33.3)	38.77
C/C	10 (38.5)	14 (45.2)	25 (75.8)	1 (33.3)	53.3
Allele frequency
A	28 (53.8)	29 (46.8)	14 (21.2)	3 (50.0)	78.09
C	24 (46.2)	33 (53.2)	52 (78.8)	3 (50.0)	103.68

TNM: Tumor-node-metastasis.

Citation: Chen RY, Cao JJ, Chen J, Yang JP, Liu XB, Zhao GQ, Zhang YF. Single nucleotide polymorphisms in the CDH17 gene of colorectal carcinoma. World J Gastroenterol 2012; 18(48): 7251-7261
URL: https://www.wjgnet.com/1007-9327/full/v18/i48/7251.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i48.7251