Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time

doi:10.3748/wjg.v18.i48.7194

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 28, 2012; 18(48): 7194-7200
Published online Dec 28, 2012. doi: 10.3748/wjg.v18.i48.7194

Table 1 Analysis of establishment of models

Group	Warm ischemia time (min)	Anhepatic phase (min)	Operative duration for biliary external drainage (min)	1-wk survival rate (%)
I	3.1 ± 0.5	18.1 ± 1.2	6.2 ± 1.2	100
II	3.0 ± 0.4	17.6 ± 1.5	7.1 ± 1.4	100
III	3.3 ± 0.3	18.5 ± 1.1	6.7 ± 1.6	90
IV	3.2 ± 0.5	17.9 ± 1.7	6.5 ± 2.0	60

Table 2 Effect of different secondary warm ischemia time on liver function (mean ± SD)

Liver function	Normal value	Group	6 h	24 h	3 d	7 d
ALT	5-40	I	165.71 ± 31.42	140.22 ± 21.08	24.30 ± 12.04	20.15 ± 8.12
(U/L)		II	356.15 ± 52.52^a	246.31 ± 22.93^a	46.43 ± 4.69^a	38.24 ± 3.52^a
		III	632.23 ± 32.28^b^c	444.32 ± 27.80^b^c	79.58 ± 17.93^a^c	56.12 ± 10.23^a^c
		IV	931.27 ± 20.21^b^d^e	801.81 ± 25.17^b^d^e	108.95 ± 11.81^b^d^e	71.26 ± 9.14^b^d^e
AST	8-40	I	855.11 ± 28.20	553.62 ± 17.66	82.02 ± 21.14	52.13 ± 17.26
(U/L)		II	1027.05 ± 42.02^a	837.31 ± 47.72^a	117.33 ± 30.86^a	84.24 ± 18.92^a
		III	1560.46 ± 68.39^b^c	1129.26 ± 137.09^b^c	173.48 ± 33.61^a^c	113.13 ± 25.72^a^c
		IV	2620.13 ± 123.68^b^d^e	2194.53 ± 297.70^b^d^e	250.10 ± 48.54^b^d^e	165.31 ± 41.26^b^d^e
AKP	47-185	I	124.52 ± 26.26	154.13 ± 32.32	121.22 ± 14.21	91.52 ± 11.56
(U/L)		II	206.54 ± 27.69^a	270.85 ± 28.48^a	189.74 ± 24.28^a	126.51 ± 16.15^a
		III	297.40 ± 34.14^b^c	363.36 ± 30.73^b^c	295.90 ± 22.04^b^c	163.26 ± 13.29^b^c
		IV	417.06 ± 10.82^b^d^e	445.81 ± 33.49^b^d^e	327.92 ± 12.36^b^c^e	227.84 ± 32.16^b^c^e
TB	0.3-1.2	I	0.82 ± 0.26	1.42 ± 0.27	0.82 ± 0.24	0.70 ± 0.21
(mg/dL)		II	1.13 ± 0.17^a	1.52 ± 0.10	0.93 ± 0.11	0.81 ± 0.16
		III	2.12 ± 0.20^a^c	2.54 ± 0.19^a^c	1.37 ± 0.20^a^c	1.04 ± 0.18^a^c
		IV	2.64 ± 0.28^b^c^e	3.43 ± 0.19^b^d^e	2.34 ± 0.27^b^c^e	1.96 ± 0.28^b^c^e
DB	0.1-0.4	I	0.83 ± 0.17	1.06 ± 0.11	0.64 ± 0.11	0.52 ± 0.21
(mg/dL)		II	1.02 ± 0.11	1.33 ± 0.12^a	0.83 ± 0.12	0.71 ± 0.16
		III	2.05 ± 0.22^a^c	2.36 ± 0.20^a^c	1.45 ± 0.22^a^c	1.32 ± 0.19^a^c
		IV	2.36 ± 0.12^b^c^e	2.75 ± 0.12^a^c^e	2.23 ± 0.20^b^d^e	2.02 ± 0.26^b^d^e

^aP < 0.05,

^bP < 0.01 vs group I;

^cP < 0.05,

^dP < 0.01 vs group II;

^eP < 0.05 vs group III. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; AKP: Alkaline phosphatase; TB: Total bilirubin; DB: Direct bilirubin.

Table 3 Effect of different timing of secondary warm ischemia on apoptosis index of bile duct epithelia and bile duct injury severity score (mean ± SD)

Group	Apoptosis index of bile duct epithelia		Bile duct injury severity score
Group	6 h	24 h	6 h	24 h
I	5.87 ± 0.50	7.13 ± 0.60	2.5 ± 0.3	2.9 ± 0.2
II	7.58 ± 0.31^a	8.96 ± 0.67^a	3.6 ± 0.3^a	3.7 ± 0.4^a
III	8.98 ± 0.69^a^c	9.90 ± 0.48^a^c	4.7 ± 0.2^a^c	5.1 ± 0.3^a^c
IV	11.99 ± 0.49^b^c^e	13.08 ± 0.65^b^d^e	6.1 ± 0.4^b^e	6.5 ± 0.2^b^c^e

^aP < 0.05,

^bP < 0.01 vs group I;

^cP < 0.05,

^dP < 0.01 vs group II;

^eP < 0.05 vs group III.

Citation: Zhu XH, Pan JP, Wu YF, Ding YT. Establishment of a rat liver transplantation model with prolonged biliary warm ischemia time. World J Gastroenterol 2012; 18(48): 7194-7200
URL: https://www.wjgnet.com/1007-9327/full/v18/i48/7194.htm
DOI: https://dx.doi.org/10.3748/wjg.v18.i48.7194