Protective role of metalloproteinase inhibitor (AE-941) on ulcerative colitis in rats

Table 1 Oligonucleotide of primers of target genes

mRNA species	mRNA		PCR product (bp)
MMP-2	Sense	5’-ACCATCGCCCATCATCAAGT-3’	348
	Antisense	5’-CGAGCAAAAGCATCATCCAC-3’
MMP-9	Sense	5’-CCCTGCGTATTTCCATTCAT-3’	600
	Antisense	5’-ACCCCACTTCTTGTCAGCGTC-3’
β-actin	Sense	5’-AAGCCTAAGGCCAACCGTGAAAAG-3’	241
	Antisense	5’-TCAATGAGGTAGTCTGTCAGGT-3’

MMP: Metalloproteinase; PCR: Polymerase chain reaction.

Table 2 Disease activity index and colonic mucosa damage index of three groups on different time (mean ± SD)

Time	Control group		Model group		AE-941 group
	DAI	CMDI	DAI	CMDI	DAI	CMDI
7th day	0.06 ± 0.14	0.00 ± 0.00	2.89 ± 0.00a	4.83 ± 0.55a	2.76 ± 0.32	4.74 ± 0.39
21st day	0.00 ± 0.00	0.00 ± 0.00	2.09 ± 0.25a	3.03 ± 0.42a	1.55 ± 0.28c	2.08 ± 0.46c
56th day	0.00 ± 0.00	0.00 ± 0.00	1.52 ± 0.30a	1.60 ± 0.35a	0.59 ± 0.19c	0.86 ± 0.37c

^aP < 0.05 vs control group;

^cP < 0.05 vs model group. DAI: Disease activity index; CMDI: Colonic mucosa damage index.

Table 3 Colonic mucosal mRNA expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 (mean ± SD)

Time	Control group		Model group		AE-941 group
	MMP-2	MMP-9	MMP-2	MMP-9	MMP-2	MMP-9
7th day	0.0062 ± 0.0017	0.0068 ± 0.0096	0.8563 ± 0.1132a	0.9936 ± 0.1187a	0.7509 ± 0.0693	0.8375 ± 0.1054
21st day	0.0056 ± 0.0012	0.0071 ± 0.0134	0.6048 ± 0.0522ae	0.6873 ± 0.0472ae	0.3983 ± 0.0218c	0.5179 ± 0.0305c
56th day	0.0058 ± 0.0015	0.0069 ± 0.0011	0.4163 ± 0.0330ag	0.4328 ± 0.0257ag	0.1093 ± 0.0072c	0.2673 ± 0.0210c

^aP < 0.05 vs control group;

^cP < 0.05 vs model group;

^eP < 0.05 vs day 7 timepoint;

^gP < 0.05 vs day 21 timepoint. MMP: Matrix metalloproteinase.

Table 4 Colonic mucosal protein expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 (mean ± SD)

Time	Control group		Model group		AE-941 group
	MMP-2	MMP-9	MMP-2	MMP-9	MMP-2	MMP-9
7th day	0.0053 ± 0.0037	0.0073 ± 0.0015	0.0986 ± 0.0084a	0.0916 ± 0.0077a	0.0923 ± 0.0071	0.0893 ± 0.0075
21st day	0.0047 ± 0.0027	0.0067 ± 0.0011	0.0773 ± 0.0052a	0.0748 ± 0.0067a	0.0536 ± 0.0034c	0.0554 ± 0.0061c
56th day	0.0057 ± 0.0026	0.0070 ± 0.0013	0.0453 ± 0.0036a	0.0537 ± 0.0035a	0.0324 ± 0.0025c	0.0364 ± 0.0025c

^aP < 0.05 vs control group;

^cP < 0.05 vs model group. MMP: Matrix metalloproteinase.