An end-to-end anastomosis model of guinea pig bile duct: A 6-mo observation

Table 1 Immunohistochemical grading scale

0	No evidence
1	Occasional evidence
2	Light scattering
3	Abundant evidence
4	Prominent distribution

Modified from[13] for the immunohistochemical grading of proliferating cell nuclear antigen.

Table 2 Body weight of guinea pigs

Groups	n	mean ± SE (g)	P
Group 1	7	380.86 ± 3.04
Group 2	8	871.38 ± 15.23	0.0021b
Group 3	7	963.00 ± 39.90	0.0021b
Group 4	7	1101.71 ± 31.96	0.0021b

¹Mann-Whitney U test;

^bP < 0.01 vs group 1.

Table 3 Measurement of common bile duct in guinea pigs

Groups	n	MDA (mm)		MDP (mm)		DR
		mean ± SE	P	mean ± SE	P	mean ± SE	P
Group 1	7	1.71 ± 0.17		2.95 ± 0.15		1.89 ± 0.27
Group 2	8	3.51 ± 0.12	0.0001b	10.24 ± 0.48	0.0012	2.94 ± 0.17	0.0041b
Group 3	7	3.15 ± 0.23	0.0001b	8.66 ± 0.47	0.0022	3.24 ± 0.65	0.0791
Group 4	7	3.47 ± 0.16	0.0001b	8.50 ± 0.52	0.0022	2.14 ± 0.18	0.4402

¹Student’s t test;

²Mann-Whitney U test;

^bP < 0.01 vs group 1. MDA: Maximum diameter of anastomosis; MDP: Maximum diameter of the proximal end; DR: Diameter ratio.

Table 4 Semi-quantitative analysis of proliferating cell nuclear antigen expression in bile duct of guinea pigs

Groups	n	mean ± SE	t	P
Group 1	7	1.57 ± 0.535
Group 2	8	3.63 ± 0.518	-7.511	0.000b
Group 3	7	3.14 ± 0.378	-6.351	0.000b
Group 4	7	1.71 ± 0.488	-0.522	0.611b

^bP < 0.01 vs group 1.

Table 5 Serum alkaline phosphatase and γ-glutamyltransferase levels in guinea pig (U/L)

Groups	n	ALP (U/L)		GGT (U/L)
		mean ± SE	P	mean ± SE	P
Group 1	6	53.33 ± 8.44		14.67 ± 2.76
Group 2	8	76.63 ± 9.95	0.1451	13.00 ± 1.04	0.5602
Group 3	7	63.29 ± 5.18	0.4241	14.00 ± 1.50	0.8291
Group 4	7	62.00 ± 13.22	0.6911	16.43 ± 1.04	0.7742

¹Student’s t test;

²Mann-Whitney U test. ALP: Alkaline phosphatase; GGT: γ-glutamyltransferase.

Table 6 Bile contents in guinea pigs

Groups	n	TBIL (μmol/L)		TBA (μmol/L)		Ca2+ (mmol/L)		pH
		mean ± SE	P	mean ± SE	P	mean ± SE	P	mean ± SE	P
Group 1	7	32.44 ± 3.43		11 668.38 ± 1549.42		0.78 ± 0.06		8.87 ± 0.07
Group 3	7	28.5 ± 7.92	0.6221	15 981.67 ± 2295.02	0.1361	1.17 ± 0.13	0.0141	8.86 ± 0.08	0.9081
Group 4	7	39.19 ± 9.97	0.5652	18 260.25 ± 4164.40	0.1641	1.24 ± 0.17	0.0642	8.82 ± 0.07	0.6421

¹Student’s t test;

²Mann-Whitney U test. TBIL: Total bilirubin; TBA: Total bile acid.